Busselton Population Medical Research Foundation

1. Busselton Population Medical Research Foundation

3. Vancouver

4. Calgary

5. London

7. Where is Busselton? The Shire of Busselton is the target population of the Busselton Health Study

8. Cross-sectional & longitudinal population health surveys, initiated in 1966 by Dr Kevin Cullen Busselton Health Studies

9. Aims As stated in 1966: 1. To study the prevalence of common diseases in an Australian community 2. To assess the range and variation of a large number of clinical and laboratory variables in a natural population • To provide a community service in the detection, treatment and prevention of disease and in the education of the population • To conduct longitudinal surveys for the study of risk factors related to health and disease • To study mortality from specific diseases in an Australian population and to define the usefulness of risk factors in predicting such mortality

11. FEV1 & FVC Airway Hyper-Responsiveness (AHR) Respiratory Symptoms Methods used in Busselton:1966 - 2007 Standardised Questionnaires Spirometry Bronchial challenges Histamine Methacholine Skin Allergy Tests Dust mites Grasses Cat/dog Mould Atopy

12. Dataset • Lifestyle & Behaviour • Nutrition • Alcohol & Tobacco use • Exercise • Mortality & Hospital Admissions • Linkage via WA Hospital Morbidity Data System • Family data • 2000 families Over 16, 000 participants since 1966 Biological specimens • Sera • DNA Physical data • Anthropometry • Lung Function • Atopy & allergy • Cardiovascular function • Mental health & cognition

13. Recent Projects Burden Of Lung Disease (BOLD) (2007-08) Asthma Prevalence Study (2005-07) 2929 adults 1500 school-aged children • 629 Busselton Adults • > 40 yrs of age Obesity, Metabolic Syndrome, CVD Risk Study (2006-08) Sleep-Disordered Breathing Study (2007- ongoing) 500 children & adolescents 1200 adults >800 adults

14. Current Projects • Regional comparision with Fremantle Diabetes Study (FDS II) • 250 Diabetics and 250 age-matched controls The Busselton Diabetes Study (BDS) (2008 - 2009) • Anthropometry • Biochemistry • Neurological assessments • Vision • Blood pressure • Brachial-Ankle Index • ECG • Spirometry • Oximetry • Sleep Apnoea • Diet & Nutrition • Quality of care • Lifestyle & well-being

15. Current Projects • Multidisciplinary focus on diseases and factors common to ageing • 3000 “Baby Boomers” 45 – 64 year olds The Busselton Healthy Ageing Study (BHAS) (Commencing 2009) • Anthropometry • DEXA scans – bone, muscle, fat • Cardiovascular profiling • Muscle strength & balance • Physical Activity • Eyes & vision • Ears & hearing • Respiratory function & health • Cognition • Diet & nutrition • Mental health & well-being • Biochemistry & DNA

16. Gene Chromosome location Variation surveyed Phenotype Assoc’n Population- analysis Cases /controls DPP10 2q14 SNPs Asthma Y A?B - TDT 244 CCR5 3p21 Δ32 Asthma N A/B - TDT 217 IL4 5q23 -589C/T Specific IgE HDM Y Pop’n based 1004 LTA 6p21 Ncol int 1 Asthma Y ? 92/318 “ “ Haplotypes Asthma Y ? 179/825 TNF 6p21 -308G/A Asthma Y ? 92/318 “ “ Haplotypes Asthma Y Pop’n based 1004 HLA-DRB1 6p21 SNP alleles Total IgE Y Quant. phenot 1004 “ “ “ “ Y Aab. – Quant. phenotype 234 HLA 6p21 Haplotypes Asthma Y ? 179/825 NOD1 7p15 In/del int 9 Total IgE Y ? ? FCER1B 11q12 Leu18/ /Leu183 Atopy SPT/IgE Y Pop,n based 1004 “ “ Gly237Glu SPT, IgE, BHR Y ? ? “ “ Rsalin 2 Asthma, total IgE Y Quant. phenot 123 “ “ Rsalex 7 Total IgE, eos Y Quant. phenot 121 PHF11 13q14 SNPs, haplotypes Total IgE, asthma Y ? ? TCRA/D 14q11 VA8.1 IgE to Der p 2 Y Pop,n based 410, 426 Genetic Studies (Asthma and Allergy) Using Busselton Data – Association Studies - Cookson (London), Palmer and others.

17. BPMRF Investigators

18. Strengths: Numbers, Longitudinal + Cross-sectional, Phenotypes (Q, airway function, blood), Family linkage. Resources: Genotyping, Staff, Infrastructure (some). Collaborative areas: Respiratory and Allergy, CVD, Diabetes

19. British Columbia

20. Western Australia