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CFSE

a. +Th17 IL-1 b. +Th17 TGF- b 1. B16-ova. EL-4. -Glu +Pyru media. Complete media. B16-ova. CFSE gated. EL-4. CD25. No T cells. 7AAD. CFSE. +Th17 TGF- b 1. +Th17 IL-1 b. Annexin V. CD69. +Th17 IL-1b (- Glu + Pyru ). c. +Th17 IL-1b (complete media). b. B16-ova. EL-4.

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CFSE

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  1. a +Th17IL-1b +Th17TGF-b1 B16-ova EL-4 -Glu +Pyru media Complete media B16-ova CFSE gated EL-4 CD25 No T cells 7AAD CFSE +Th17TGF-b1 +Th17IL-1b Annexin V CD69 +Th17IL-1b (-Glu +Pyru) c +Th17IL-1b (complete media) b B16-ova EL-4 B16-ova CFSE gated EL-4 No T cells CFSE +Th17IL-1b (complete media) 7AAD +Th17IL-1b (-Glu +Pyru) Annexin V Supplementary figure 1

  2. Th17IL-1b a -Glu +pyru Complete media Th17TGF-b1 Nt5e (CD73) Entpd1 (CD39) c Mice#2 Mice#1 Mice#3 CD39 Th17TGF-b1 CD73 No transfer Th17IL-1b (complete media) Th17TGF-b1 Th17IL-1b (-Glu +Pyru) Th17IL-1b b Th17TGF-b1 transfer CD4 Vb5.1 Th17IL-1b+TGF-b transfer Supplementary figure 2

  3. a Supplementary figure 3

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