1 / 8

CFSE

9. 9. 5. 10. 4. 10. 3. 10. 2. 10. 0. 7. 75. 5. 3. 4. 0. 10. 10. 10. 5. 5. 5. 10. 10. 10. 4. 4. 4. 10. 10. 10. 3. 3. 3. 10. 10. 10. 0. 0. 0. 2. 2. 2. 3. 3. 3. 4. 4. 4. 5. 5. 5. 0. 0. 0. 10.

kirra
Download Presentation

CFSE

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 9 9 5 10 4 10 3 10 2 10 0 7 75 5 3 4 0 10 10 10 5 5 5 10 10 10 4 4 4 10 10 10 3 3 3 10 10 10 0 0 0 2 2 2 3 3 3 4 4 4 5 5 5 0 0 0 10 10 10 10 10 10 10 10 10 10 10 10 Figure 1 B C D Total Total A CD62L CD62L CD69 CD69 CD103+ ICOS+ CD103+ ICOS- CD103 CD103 CD103- ICOS+ CD103- ICOS- 5 6 8 4 ICOS ICOS CD103 3 4 5 4 85 77 11 CD127 0 10 10 10 CD69 CD62L FOXP3 CFSE CD127 2 10 2 11 5 10 4 10 3 10 0 1 85 3 86 CD103 2 3 4 5 0 10 10 10 10 ICOS 2 2 2 2 2 3 3 3 3 3 4 4 4 4 4 5 5 5 5 5 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 ICOS 0 0 0 0 0

  2. Figure 2 600 500 A Ex vivo Activated 400 CD127 MFI 300 200 100 0 FOXP3- FOXP3+ 8,4 2,6 0,2 3,2 5 B C 5 ns 10 10 * * FOXP3 FOXP3- ICOS 3 4 5 0 10 10 10 4 FOXP3+ 4 10 10 CD127 Ex vivo Activated 3 3 10 10 0 0 83,3 5,7 31,7 64,9 0 3 4 5 3 4 5 0 10 10 10 10 10 10

  3. Figure 3 Total CD4+ CD25+ A B ns ** ** CD45.1+ FOXP3- 400 300 CD127 MFI CD45.1- FOXP3+ 200 100 0 10 10 10 10 CD45.1+ FOXP3- CD45.1- FOXP3+ CD127 "Naive-like" CD4+ CD25+ D C ns ** * CD45.1+ FOXP3- 400 0 2 3 4 5 10 10 10 10 300 CD127 MFI CD45.1- FOXP3+ 200 100 0 CD127 CD45.1+ FOXP3- CD45.1- FOXP3+ 2 3 4 5 0 Ex vivo Transferred

  4. 5 5 10 10 4 4 10 10 3 3 10 10 0 0 0 2 3 4 5 10 10 10 10 6 4 2 0 CTRL DNFB 1 0,8 0,6 0,4 0,2 0 CTRL DNFB 15 21 58 24 5 10 4 10 3 10 0 22 42 8 10 Figure 4 32 47 A B ** ** 18 3 2 3 4 5 0 10 10 10 10 CD4 T cells absolute numbers (106) in dLN Ear Thickness (mm) ICOS C Draining Lymph Nodes CD127 CTRL DNFB 9 4 1 1 ICOS- ICOS+ CD4+ FOXP3- 28 68 5 22 76 15 72 10 23 24 10 9 CD4+ FOXP3+ 4 10 14 67 37 16 CD127 0 2 3 4 5 10 10 10 10 3 0 0 0 0 2 2 2 2 3 3 3 3 4 4 4 4 5 5 5 5 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 Ear skin D 10 ICOS CTRL DNFB CD127 ICOS- ICOS+ 0 CD4+ FOXP3- 2 2 2 3 4 5 0 CD4+ FOXP3+ 10 10 10 10 CD127

  5. 10.5 5.5 5.5 0.1 5 5 10 10 4 4 10 10 3 3 10 10 0 0 89.1 21.6 67.4 0.3 FOXP3 FOXP3 FOXP3 FOXP3 CD127 Isotype Control CD127 Isotype Control Figure 5 A PC LN Intestine SP 8.8 8.1 2.5 0.1 5 5 10 10 1.7 0.1 9.0 9.3 5 5 10 10 4 4 10 10 4 4 10 10 1.5 0.2 40.5 12.7 8.9 9.8 25.0 0.3 3 3 5 5 10 10 10 10 3 3 0 0 10 10 5 5 Lung 0 0 27.9 91.0 61.6 0.1 4 4 PB 10 10 63.6 1.0 89.9 25.4 10 10 7.7 8.5 3 3 0.1 1.8 5 5 10 10 10 10 0 0 36.7 0.6 90.2 52.0 4 4 10 10 1.8 0.0 1.8 0.2 5 5 10 10 38.8 6.7 30.7 0.3 3 3 10 10 2.9 3.8 0.1 1.0 Liver 4 4 5 5 10 10 10 10 0 0 Thymus 5 5 91.1 33.9 56.6 0.3 4 4 4 4 3 3 10 10 10 10 10 10 4.7 7.3 0.0 0.4 5 5 0 10 10 0 10 10 97.8 0.4 61.0 37.0 3 3 10 10 4 4 0 0 10 10 95.2 62.0 34.1 0.9 Skin 3 3 10 10 BM 0 0 68.4 94.8 23.9 0.5 4 4 3 3 10 10 10 10 B C 0 0 500 100 FOXP3 - 400 80 FOXP3 + 34.1 0.3 58.9 28.2 300 60 % CD127 high Tregs 3 3 CD127 MFI 40 200 10 10 100 20 3 3 4 4 5 5 0 0 0 0 PC PB LN PC SP PB BM SP BM Lung Lung Skin LN Liver Liver Skin 0 0 10 10 10 10 10 10 Intestine Thymus Intestine Thymus 59.6 21.5 42.1 1.3 3 3 4 4 5 5 0 0 10 10 10 10 10 10

  6. Figure 6 A B FOXP3- CD127lo FOXP3+ CD127hi NT IL-2 IL-7 0.1 IL-7 1 IL-7 10 pSTAT5 pSTAT5 C D FOXP3- LN+SP BM FOXP3+ NT IL-7 0.1 IL-7 10 0 0 0 0 pSTAT5 pSTAT5 2 2 2 2 3 3 3 3 4 4 4 4 5 5 5 5 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10

  7. CD25 CD127 Figure 7 A L H 5 10 B *** ** 40 4 *** 10 30 * 3 20 Live cells (%) 10 10 0 0 Total CD127 Low CD127 High 2 3 4 5 0 10 10 10 10

  8. Supp Figure 1 A B FOXP3- CD127lo FOXP3+ CD127hi NT IL-2 TSLP 1 TSLP 10 TSLP 100 pSTAT5 pSTAT5 C D FOXP3- LN+SP BM FOXP3+ NT TSLP 100 pSTAT5 pSTAT5

More Related