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Effects of Fusion Partners on Protein Solubility in DXS: A Study

This study explores the impact of fusion partners (trxA, nusA, malE, slyD) on the solubility of DXS protein. Using an araBAD promoter-DH10B strain at 20°C, the study found no significant improvement. Data presented are averages of triplicates with standard errors plotted.

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Effects of Fusion Partners on Protein Solubility in DXS: A Study

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  1. N. D. p > 0.1 Supplementary figure S5 Effects of fusion partners on DXS solubility Fusion partners were reported to increase solubility of recombinant proteins. Four frequently used fusion partners (trxA, nusA, malE and slyD) were fused at N-terminus of DXS in this study in the attempt to increase protein solubility of DXS. The expression system (araBAD promoter-DH10B strain) and the temperature (20 °C) which produced most soluble DXS were used in this study. It was found that the protein solubility of DXS was not significantly improved by the use of the fusion partners. Presented data were average of triplicates and standard errors were drawn on the plot. N.D.: Not detected. Student’s t-test was used to calculate the p values in the statistical analysis.

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