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Colon NAT. Colon NAT. Tissue pool. Tissue pool. Colon lines. Colon TUM. Colon TUM. Colon lines. NTC. NTC. hsa-mir-92b. hsa-mir-594. hsa-mir-565. hsa-mir-600. hsa-mir-574. hsa-mir-625. hsa-mir-580. hsa-mir-628. hsa-mir-592. hsa-mir-548d-2.
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Colon NAT Colon NAT Tissue pool Tissue pool Colon lines Colon TUM Colon TUM Colon lines NTC NTC hsa-mir-92b hsa-mir-594 hsa-mir-565 hsa-mir-600 hsa-mir-574 hsa-mir-625 hsa-mir-580 hsa-mir-628 hsa-mir-592 hsa-mir-548d-2 Figure 7. qRT-PCR expression validation of miRNA candidates. Expression of miRNAs was analyzed in total RNA derived from colon tumor tissue (TUM); adjacent normal colonic epithelial tissue (NAT); pooled colorectal tumor cell lines HCT116, DLD-1, and RKO (Colon lines); pooled extra-colonic tissue from brain, cervix, thymus, and skeletal muscle (Tissue pool); and a no template control (NTC). The lower band present in all NTC lanes represents primer dimers. miR-24 5S rRNA