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Shift in Adaptive Immune Response Types in Dry-Eye Disease

Shift in Adaptive Immune Response Types in Dry-Eye Disease. Yihe Chen, Sunil K Chauhan, Reza Dana Schepens Eye Research Institute Harvard Medical School Boston, MA. The authors have no financial interest in the subject matter of this poster. Background. Dry eye disease (DED)

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Shift in Adaptive Immune Response Types in Dry-Eye Disease

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  1. Shift in Adaptive Immune Response Types in Dry-Eye Disease Yihe Chen,Sunil K Chauhan, Reza Dana Schepens Eye Research Institute Harvard Medical School Boston, MA The authors have no financial interest in the subject matter of this poster

  2. Background • Dry eye disease (DED) • An inflammatory autoimmune disorder • T cell involvement in the pathogenesis Characterization of adaptive immune response during DED course would be relevant to the development of more effective therapies

  3. Purpose Determine kinetics of generation of adaptive immunity in DED

  4. Methods DED induction [Controlled-environment chamber] 2 or 7 days Conjunctiva Tissue harvest Cervical lymph nodes Analysis of immune response PCR FCM

  5. Results IFN-γ and IL-17 mRNA levels Both the conjunctiva and draining lymph nodes of DED mice showed an enhanced expression of IFN-γ mRNA on day 2 while a substantially increased expression of IL-17 mRNA on day 7 Conjunctiva Draining lymph nodes * * * * NL NL NL: mRNA level in normal mice; * p < 0.05 compared with normal

  6. Results Th1 and Th17 cells • Th1 cells: significantly increased on day 2, and then diminished to the baseline level on day 7 • Th17 cells: no significant change on day 2, but significantly increased on day 7 Th1 Day 2 Day 7 IFN-γ * Th17 IL-17 * CD4 Th1/17 cell is defined as IFN-γ+ / IL-17+CD4+ population; * p < 0.05 compared with normal

  7. Conclusion • These findings suggest: • the adaptive immune response in early DED is characterized by a Th1 phenotype, whereas, • the progression of DED is characterized by the Th17 response

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