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Molecular Diagnosis of GI Cancer 胃肠道肿瘤分子诊断进展

Molecular Diagnosis of GI Cancer 胃肠道肿瘤分子诊断进展. 卫生部国家临床重点专科 厦门大学附属中山医院消化内科 厦门大学消化疾病研究所 厦门市消化疾病中心 巴亚斯古楞. Summary, including clinically deployable markers and potential subtype-guided therapies for CRC. Part 1. Serum levels of TFF3 were significantly elevated

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Molecular Diagnosis of GI Cancer 胃肠道肿瘤分子诊断进展

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  1. Molecular Diagnosis of GI Cancer胃肠道肿瘤分子诊断进展 卫生部国家临床重点专科 厦门大学附属中山医院消化内科 厦门大学消化疾病研究所 厦门市消化疾病中心 巴亚斯古楞

  2. Summary, including clinically deployable markers and potential subtype-guided therapies for CRC

  3. Part 1

  4. Serum levels of TFF3 were significantly elevated in both gastric and colorectal cancer patients

  5. Urine levels of TFF3 were significantly elevated in both gastric and colorectal cancer patients

  6. Serum TFF3 levels as a prognostic marker for gastric cancer Higher serum TFF3 level was significantly correlated With the clinical stage (I-III vs IV) and distant metastasis

  7. Serum levels as a prognostic marker for colorectal cancer Higher serum TFF3 level was significantly correlated with the clinical stage (I-III vs IV) and distant metastasis. Older patients (age >60 y) were higher than the levels of; different numbers of lymphatic metastases

  8. Serum TFF3 levels as predictors of responses to chemotherapy in gastric and colorectal cancer PR patients

  9. Part 2 Serum level of JTB * P<0.0001 ng/ml * P<0.0001 Gastric cancer Colon cancer Healthy individuals

  10. Serum level of JTB 肠癌血清检测 ng/ml

  11. JTB, how to become a biomarker? Breakpoint 1q21 Chro 1 signal Transmembrane N mJTB C sJTB N C Digestive cancer patients Blood samples ELISA

  12. JTB逆转录示意图

  13. 确诊肿瘤病人外周血循环细胞JTB-DNA-逆转录条带确诊肿瘤病人外周血循环细胞JTB-DNA-逆转录条带 阳性率统计结果 100% 75% 18% Health Tumor

  14. JTB 条带位置的多样性，提示融合蛋白的存在 Hela-pu6 Hela-pu6 Hela-siJTB Hela-siJTB Hela-pu6 Hela-pu6 Hela-siJTB 135KD 105KD JTB (Novus) JTB (Mybio) 57KD 48KD 38KD 15KD tubulin

  15. 质谱鉴定结果 57KD Vimentin 38KD Unamed protein 48KD KRT8

  16. 融合基因鉴定 Hela HepG2 750bp RT-PCR JTB-F Vimentin-R 待测序报告？

  17. Part 3 Microenvironments Dev Cell. 2010 June 15; 18(6): 884–901

  18. Nature Immunology5, 88 - 97 : January 2004 The extracellular matrix protein mindin is a pattern-recognition molecule for microbial pathogens Department of Immunology, Duke University Medical Center, Durham, Generation of Mindin-deficient mice

  19. Decreased serum Mindin levels can be used as a biomarker for the early detection of colorectal cancer

  20. ROC curve analysis 86.78% sensitivity and 81.25% specificity in separating stage I-II patients and 51.85% sensitivity and 80.21% specificity in separating stage III-IV patients from the healthy controls. Mindin had 76.15% sensitivity and 81.31% specificity in separating colorectal cancer patients from the normal controls.

  21. Mindin was significantly increased in the PR group after chemotherapy, and its increase was identified as a predictor for chemotherapy response in the PR group

  22. cancer EGR1 EGR1 MindinExpression Mindin promoter cgcccccgc

  23. Results

  24. CH-IP EGR1 Mindin Western blot Western blot RT-PCR RT-PCR 血清学检测 组织中表达 组织中表达 报告基因 Western blot 分子机制 信号通路 Future plan

  25. Our Goal Combination of TFF3, JTB and Mindin for Early Diagnosis of GI Cancer!

  26. 我力有限，靠你众力搏程；我力有限，靠你定向领航；遥遥苦辛路，浓浓同路情；为了那一天，启程－－我力有限，靠你众力搏程；我力有限，靠你定向领航；遥遥苦辛路，浓浓同路情；为了那一天，启程－－

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