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A. -. -. -. wild-type. wild-type. wild-type. V9I F86Y. V9I F86Y. V9I F86Y. F67S F86Y. F67S F86Y. F67S F86Y. F86Y G138D. F86Y G138D. F86Y G138D. V9I F86Y G138D. V9I F86Y G138D. V9I F86Y G138D. rtTA. rtTA. rtTA. F86Y. F86Y. F86Y.
E N D
A - - - wild-type wild-type wild-type V9I F86Y V9I F86Y V9I F86Y F67S F86Y F67S F86Y F67S F86Y F86Y G138D F86Y G138D F86Y G138D V9I F86Y G138D V9I F86Y G138D V9I F86Y G138D rtTA rtTA rtTA F86Y F86Y F86Y sc-rtTA with mutations in the N-terminal TetR domain Transcriptional activity (%) B sc-rtTA with mutations in the C-terminal TetR domain Transcriptional activity (%) C sc-rtTA with mutations in both TetR domains Transcriptional activity (%)