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Apple Enzyme Kinetics. What is “Kinetics”?. Determined by: Represented by . Activation Energy, E a. What can effect. E act 1. E act -1. Collisions. Reactants must __________________________ for a reaction to occur What can affect the rate of collisions?. Speed of Collisions.
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What is “Kinetics”? • Determined by: • Represented by
Activation Energy, Ea • What can effect Eact1 Eact-1
Collisions • Reactants must __________________________ for a reaction to occur • What can affect the rate of collisions?
What is a Catalyst? • a substance that • The rate is usually increased • This is accomplished by • Cannot make a reaction occur that • Only changes how a reaction can occur
Examples of Catalysts • Acids • Bases • Metals • Enzymes
What are Enzymes? • Enzymes are a specific type of • Proteins are • Proteins are what your provides the codes to make • catalysts • Enzymes are that reactions in systems
Lock and Key Model Induced Fit Model How Do Enzymes Work? Enzyme Substrate Product
What Reaction Will We Be Studying? • This reaction occurs • The natural reaction rate is so • RUN A NEGATIVE CONTROL WITH JUST CATECHOL AND WATER • MEASURE THE ABSORBANCE AND RECORD THE TIME • MEASURE THE ABSORBANCE AND TIME AFTER ALL OF THE OTHER EXPERIMENTS • CALCULATE (ABS2-ABS1)/(TIME2-TIME1) = RATE + ½ O2 + H2O
Your Goals • You need to indicate • You accomplish this by plotting and • We will collect class data BEFORE YOU LEAVE THE LAB
Broccoli Experiment Starts Today • Begin to do this BEFORE the apple experiment • We just need to dry the broccoli today • Clean a crucible • Weigh broccoli (15g) in crucible • Heat on a hot plate to dehydrate • May have to mix occasionally • Store in personal drawer until next lab • HAVE A GREAT VACATION!!!