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Syed Lab Weekly MEA meeting Session 2. DEP experiment plans

Syed Lab Weekly MEA meeting Session 2. DEP experiment plans. J. James Jun Physics Honours University of Calgary Feb 9, 2007. Organization of this presentation. Phase I. DEP Experiment with microsphere. Phase II. Tracking & Guiding Software. Phase III. DEP Experiment with Neuron.

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Syed Lab Weekly MEA meeting Session 2. DEP experiment plans

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  1. Syed Lab Weekly MEA meeting Session 2. DEP experiment plans J. James Jun Physics Honours University of Calgary Feb 9, 2007

  2. Organization of this presentation Phase I. DEP Experiment with microsphere Phase II. Tracking & Guiding Software Phase III. DEP Experiment with Neuron

  3. DEP experiment with MicrosphereMovement control on Single Grid Goal To demonstrate the proof of principle To find optimum nDEP frequency & amplitude Procedures 1. Isolate individual Microsphere 2. Handle with pulling pipette 3. Place onto MEA 4. Apply AC signal on MEA 5. Record a movie

  4. DEP with MicrosphereMovement control on Dual Grids Goal To pass a microsphere between grids Procedures 1. Locate a microsphere on Grid 1 2. Apply nDEP until bead reaches the centre 3. Turn off the nDEP 4. Wait until the microsphere moves to Grid 2 5. Turn on the nDEP field on the Grid 2

  5. Tracking & Guidance Software Goal To track a movement of microsphere To guide a movement of microsphere Development Plan Fast image acquisition Object tracking w/ Fluorescent imaging Function generator controlling software

  6. Tracking & Guidance SoftwareFront End

  7. Tracking & Guidance SoftwareBack End Camera Vision Tracking Motion Coordinator Function Gen. Electrode Selector Legend: Thread Device

  8. Summary of my work on December & January Questions?

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