1 / 10

海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF)

海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF). 指導老師：詹于誼 組員：楊仲智 張芳瑛 翁曼真. 0.2 to 4 m M using a continuous 96 hr(median = 0.75 Mm ). Images of cells treated with KF. control. KF. 0.5 m M for HepG2. 5 m M for PLC/PRF/5C. 0.1 m M. 0.5 m M.

kort
Download Presentation

海洋天然物 Elysia rufescens 海蛞蝓 Kahalalide F (KF)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 海洋天然物Elysia rufescens 海蛞蝓 Kahalalide F (KF) 指導老師：詹于誼 組員：楊仲智 張芳瑛 翁曼真

  2. 0.2 to 4 mM using a continuous 96 hr(median = 0.75 Mm).

  3. Images of cells treated with KF control KF 0.5 mM for HepG2 5 mM for PLC/PRF/5C

  4. 0.1mM 0.5mM 1mM 2.5mM 5mM 0.5–0.7 mM KF in HepG2 cells KF-induced depletion of ATP in HepG2 and PLC/PRF/5C cells 1mM 2.5mM 5mM 10mM 25mM 4–6 mM KF in PLC/PRF/5C cell

  5. Analysis of KF-induced permeability changes in HepG2 cells by flow cytometry (a) control

  6. (b) treated with 0.25 mM KF for 1 h

  7. LDH release by HepG2 and PLC/PRF/5C after exposure to KF for 1 h. HepG2 PLC/PRF/5C

  8. Discussion • HepG2 cells demonstrated profound ATP depletion, associated with cell swelling and cell blebbing, and increased permeabilityto propidium iodide (PI), annexin V (AV) and release of lactate dehydrogenase (LDH).cell membrane induced by KF in HepG2 • PLC/PRF/5C cells retained their cellstructure, but were permeable to PI and, following exposure to high concentrations of KF, to AV. PLC/PRF/5C suggest specific interactions with membranes or proteins. • This could lead to better drug design aimed at exploitingthe potential for cell selectivity.

  9. Thanks for your attention

More Related