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Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods. Beatriz Díez 1 , Terence L. Marsh 2 , Ramon Massana 1 and Carlos Pedrós-Alió 1

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Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods

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  1. Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods Beatriz Díez1, Terence L. Marsh2 , Ramon Massana1 and Carlos Pedrós-Alió1 1Departament de Biologia Marina, Institut de Ciències del Mar, CSIC, Passeig Joan de Borbó s/n, E-08039 Barcelona, Catalunya, Spain 2Center of Microbial Ecology and Department of Microbiology, 41 Gilner Hall, Michigan State University, East Lansing, 48824 Michigan, USA

  2. Southern Ocean: area studied

  3. DHARMA cruise DOVETAIL cruise

  4. Objectives Study the distribution and variability of eukaryotic picoplankton in Southern Ocean Comparison of DGGE and T-RFLP results Identification of the dominant populations by both techniques Emphasis on the distribution of novel phylogenetic groups: stramenopiles and alveolates

  5. ICE-EDGE DOVETAIL transect DHARMA transect EDGE-ICE

  6. DOVETAIL Surface T-RFLP (MspI) Mantoniella DOV1 DOV2 DOV3 DOV4 Phaeocystis DOV5 DOV6

  7. DOVETAIL transect DGGE T-RFLP (MspI and RsaI) ICE-EDGE ICE -EDGE A=SURFACE B=Bottom of mixed layer

  8. 19** 18 17** 15 16 14 10 13 9 12 11 8 7* 6 5 4 3 2 1

  9. Edge-ice SAF DH32 DH12 DH18 DH24 DH1 Dharma 5 - 0.2µm DH1= Ice-edge DH12= Scotia Front DH24= Polar Front DH32= Subantartic Front

  10. DHARMA DGGE T-RFLP (MSPI) ICE ICE WSF ICE WSF ACC ACC WSF PF PF AND SAF SAF

  11. DHARMA CYTOMETRY DH11 --- +----- DH14 --- | +----- DH18 -- | | +-- | | DH12 -- | | | +---- | DH5 - | | +- | | DH9 -| | | +-- | DH20 -- | +------------------------------- DH22 -- | +----------- DH3 -+ | DH1 -- DH24 ------ +-------------------------------------- DH28 - | | | DH26 + | | | DH32 + | +----- DH30 - PF

  12. *Novel alveolates **Novel stramenopiles

  13. DH18 (WSF) 19** 18 17** 16 10 13 12 11 8 7* 6 5 4* 10 25 60 100 250 500 1000 2000 3000 Depth (meters)

  14. Conclusions DGGE and T-RFLP fingerprinting approaches provide information about changes in population distribution. Also they offer the possibility to identify the dominant components of the natural populations. When the same size fractions were compared, the assemblages were rather similar in the two transects. Despite the high resolution of the T-RFLP technique, usually more than one species had the same restriction site in the 18S rDNA, resulting in an identical peak size. The most likely candidate must be decided with information from other techniques

  15. Although diatoms have been usually found to be dominant in Antarctic algal blooms and are the best known group, it is clear that small-celled species (<5 µm) and not only autotrophic organisms, are very important in many antarctic locations Similar phylogenetic groups and dominant species were detected by DGGE and T-RFLP. Prasinophyceae, Prymnesiophyceae, Bacillariophyceae, Novel stramenopiles, Dinophyceae, and Novel alveolates, were found to be the most widespread groups, further supporting the suggestion that these organisms are common and widely distributed both in Antarctic waters and in most Oceans

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