1 / 6

E. coli HdeB and HdeA Plasmid Transfection Experiment Results Report by Lekiya Feng

This experiment report by Lekiya Feng details the successful selection of E. coli strains through PCR and sequencing, leading to plasmid extraction, L. lactis transformation, and phenotype confirmation. The report outlines the completion of fermentation experiments and plans for future protein electrophoresis and gene elongation studies for enhanced nisin production. The addition of a His-tag to the genes is proposed for further research to contribute to the final paper.

ksena
Download Presentation

E. coli HdeB and HdeA Plasmid Transfection Experiment Results Report by Lekiya Feng

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Experiment results Reporter: lekiya Feng

  2. E.coli hdeB hdeA P45 P45 hdeB hdeA Choose 24 strains randomly in two plates respectively. Add general premiers Colonies PCR Not yes E.coli A:417+261=678 B:415+261=676 The length of PCR product is 261

  3. Yes strains are selected by colonies PCR Sequencing Strains nourishment Yes! Complete correct ! No! experiment fails Plasmid extraction The preparation of Competent L.lactis Endonuclease: BamH1 &Hind111 Aim plasmid enters L.lactis by electrotransformation Aim stripes were located at 678 and 676 respectively. Phenotype Confirmation Screening by antibiotic erythromycin yes NO ! False phenotype Fermentation Experiments Yes! correct Colonies PCR

  4. Next schedules • Protein electrophoresis conformation • fermentation experiment to verify whether addition of the genes can elongate fermentation period and increase nisin production • More accurate experiments results which , from my point of view , could contribute to my final paper is to ligate a His-tag at one end of the genes.

  5. More details I need in order to fulfill the schedules I made in last slide.

  6. Thanks!

More Related