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Experiment results

Experiment results. Reporter: lekiya Feng. E.coli. hdeB. hdeA. P 45. P 45. hdeB. hdeA. Choose 24 strains randomly in two plates respectively. Add general premiers. Colonies PCR. Not. yes. E.coli. A:417+261=678 B:415+261=676. The length of PCR product is 261.

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Experiment results

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  1. Experiment results Reporter: lekiya Feng

  2. E.coli hdeB hdeA P45 P45 hdeB hdeA Choose 24 strains randomly in two plates respectively. Add general premiers Colonies PCR Not yes E.coli A:417+261=678 B:415+261=676 The length of PCR product is 261

  3. Yes strains are selected by colonies PCR Sequencing Strains nourishment Yes! Complete correct ! No! experiment fails Plasmid extraction The preparation of Competent L.lactis Endonuclease: BamH1 &Hind111 Aim plasmid enters L.lactis by electrotransformation Aim stripes were located at 678 and 676 respectively. Phenotype Confirmation Screening by antibiotic erythromycin yes NO ! False phenotype Fermentation Experiments Yes! correct Colonies PCR

  4. Next schedules • Protein electrophoresis conformation • fermentation experiment to verify whether addition of the genes can elongate fermentation period and increase nisin production • More accurate experiments results which , from my point of view , could contribute to my final paper is to ligate a His-tag at one end of the genes.

  5. More details I need in order to fulfill the schedules I made in last slide.

  6. Thanks!

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