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CERISE Experiment “ C.elegans RNAi space experiment”

Instructor:. Toru Mori Takashi Yamasaki. (mori.tooru@jaxa.jp) (yamasaki.takashi@jaxa.jp).

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CERISE Experiment “ C.elegans RNAi space experiment”

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  1. Instructor: Toru Mori Takashi Yamasaki (mori.tooru@jaxa.jp)(yamasaki.takashi@jaxa.jp) Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA). JEM CERISE 31407 CERISE Experiment “C.elegans RNAi space experiment”

  2. Objectives After this lesson the trainee will be able to: • CERISE Experiment • Explain the science objectives of the CERISE experiment. • List onboard outcomes from performing the CERISE experiment. • Operation Flow • Outline the CERISE operations flow from the view point of the sample movement. • Perform the CERISE Experiment Operations according to ODFs without instructor’s assistance.

  3. Lesson Outline CERISE Experiment . . . . . . . . . . . . . . . . . . 4 Operation Flow of CERISE . . . . . . . . . . . . . . . . 6 Key Points for CERISE Operations . . . . . . . . . . . 8 Appendix 1. Specification of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 11 2. Schematic of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 12 3. Photo of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 13 4. Acronyms . ... . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

  4. CERISE Experiment • CERISE Experiment (PI: Atsushi Higashitani) • Research Objectives • Investigate whether RNAi occurs in space as the same manner as on the earth. • Explore effects of microgravity on entire gene and protein expressions, and phosphorylations beyond generations. • Onboard Outcomes from the Experiment • Cultivated nematode • Dosimeter • Temp Logger • Microgravity environment data (taken by MMA) Returned Contained in Culture Bag Holder Returned Downlinked Culture Bag Holder Microgravity Measurement Apparatus (MMA)

  5. Intentionally Blank

  6. LEGEND: :Crew operations :Crew/Ground cooperative operations :Ground operations Operation Flow of CERISE CBEF ULF3 Saibo Rack/CBEF Activation Launch MMA Sample Preparation MLT and MMA Activation Meas Exp Unit A (4) Instl into CBEF If they are off Cultivation (4 days) CB Meas Exp Unit A (2) Retrieve MELFI CB Setup -80 deg C Micro-G environment measurement • Sample Retrieve • Meas Exp Unit A (1) re-installation with dummy mass CB Activation Meas Exp Unit A FLIGHT ULF3 Microscope Observation Cultivation (4 days) Stow As required Meas Exp Unit A (3) Retrieve MLT and MMA Deactivation Sample Retrieve Microscope Observation Stow Saibo Rack/CBEF Deactivation Culture Bag Holder CB Deactivation Return CB Stow ULF3(TBD)

  7. Operation Flow of CERISE (cont’d) Sample Preparation Cultivation (4 days) Sample Retrieve Microscope Observation Incubator Unit Micro-G Retrieve one Culture Bag From each Stow Attach Remove nematode nematode Culture Bag Pins Rtrv Clear Plate Stow Incubator Unit 1G Install Stow Culture Bag Holder (4) MELFI (-80 deg C) Install Rtrv • Culture Bag x 15 • Dosimeter x 1 • Temp Logger x 1 Observe nematode Rtrv nematode Stow Meas Exp Unit A (4) Stow CBEF Temp Logger Reuse Meas Exp Unit A Re-Instl with dummy mass Cultivation (4 days) CB Microscope Incubator Unit Micro-G Dummy Mass nematode Sample Retrieve Microscope Observation Reuse Culture Bag Holder (no nematode contained) Incubator Unit 1G Dummy Mass nematode Re-Instl Install CBEF

  8. Key Points for CERISE Operations (CBEF) <CBEF> NOTE: Underlined key points have been covered by CBEF Advanced Training.

  9. Key Points for CERISE Operations (CB) <CB> NOTE: Underlined key points have been covered by CB Advanced Training.

  10. Appendix • Appendix • Specification of Meas Exp Unit A • Schematic of Meas Exp Unit A • Photo of Meas Exp Unit A • Acronyms

  11. Specification of Meas Exp Unit A

  12. Schematic of Meas Exp Unit A CBEF Circuit Board Analog (A) Temperature Sensor (1) (0~5V) Jumper Relay circuit Analog (B) Temperature Sensor (2) (0~5V) Relay circuit SEL +12V Amplifier circuit +15V -15V NTSC Connector for future experiments Shield

  13. Photo of Meas Exp Unit A Inside Overview

  14. Acronyms CB CBEF MELFI MLT MMA OC RNAi Clean Bench Cell Biology Experiment Facility Minus Eighty degree Celsius Laboratory Freezer for ISS MMA Laptop Terminal Microgravity Measurement Apparatus Operation Chamber Ribonucleic Acid Interference

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