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CELLS @ WORK. Cell Metabolism and ATP. ATP is the energy molecule of the cell. Billions are used and reassembled every second!! Endergonic Rxn – Requires energy (ie. Photosynthesis) Exgergonic Rxn – releases energy (ie.Cellular Respiration) Glucose = 1 dollar ATP = 1 penny. Enzymes.
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Cell Metabolism and ATP • ATP is the energy molecule of the cell. • Billions are used and reassembled every second!! • Endergonic Rxn – Requires energy (ie. Photosynthesis) • Exgergonic Rxn – releases energy (ie.Cellular Respiration) • Glucose = 1 dollar • ATP = 1 penny
Enzymes Our “Key” to Biochemical Reactions
ENZYMES • Proteins that accelerate chemical reactions • Almost all processes in the cell need enzymes in order to occur – Cellular Respiration, Photosynthesis, food digestion etc. • Are extremely selective – very specific to certain reactions
ENZYMES • For Example: Lysozyme digests bacterial cell walls, and is found in human tears, egg-white, etc
ENZYMES • Enzymes are known to catalyze about 4,000 reactions in the human body • Named according to the reaction they catalyze … “ase” is added to the name of the substrate • Ex: Lactase breaks down lactose
HOW ENZYMES WORK: • By providing a lower activation energy for a reaction and dramatically accelerating its rate • For example… (Do not copy) • the reaction catalysed by orotidine-phosphate decarboxylase will consume half of its substrate in 78 million years if no enzyme is present. However, when the decarboxylase is added, the same process takes just 25 milliseconds
HOW ENZYMES WORK: Enzyme SUBSTRATE PRODUCT(S) Eg: Sucrase Sucrose + Water Glucose + Fructose Enzymes help a reaction to occur … without being directly involved!!!
HOW ENZYMES WORK: • “Lock and Key” Model:
Competitive Inhibitors • A molecule similar in shape to the substrate bonds with the enzyme’s active site and inhibit its function. • Poisons: cyanide and arsenic bind to key enzymes in this manner…death may result!
F.Y.I: • Carbohydrases break down ________ • Proteases break down… ________ • Lipases break down… ________
ENZYMES • Work at optimal temp. & pH • If it is too hot, an enzyme can become DENATURED… and will no longer function properly