1 / 6

Cycle

Age-induced increase in p16 INK4a expression in purified islets. p19 ARF expression in pancreatic compartment (old mice). pdx expression in pancreatic compartment (old mice). Islets. Islets. Exocrine. Old. ∆ Reaction. Exocrine. Young. Cycle. Cycle. Cycle. p16 INK4a. Arf. Pdx1. a.

lacey-bridges
Download Presentation

Cycle

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Age-induced increase in p16INK4a expression in purified islets p19ARF expression in pancreatic compartment (old mice) pdx expression in pancreatic compartment (old mice) Islets Islets Exocrine Old ∆ Reaction Exocrine Young Cycle Cycle Cycle p16INK4a Arf Pdx1 a b c Old Islet (64-80 weeks) = 16 16 Young Islet (12-14 weeks) 8 8 4 4 2 2 1 1 Relative Expression Relative Expression 0.5 0.5 p15INK4b p18INK4c p19INK4d p21CIP p27KIP 0.25 0.25 0.13 0.13 0.06 0.06 Exocrine Islet Exocrine Islet Age: 12-14 weeks 64-80 weeks Krishnamurthy et al., Supp. Figure 1

  2. a R1 R1 1a 2 3 1b Adrenal Lung Heart Pancreas Intestine Skin Kidney Spleen Liver Geometric Mean < = undetectable in wild-type Ink4a/Arf locus PI = Intron encoded nuclease PI-SceI RI = Eco RI IIII = Variable microsatellite region  = PCR screening primers PI R1 R1 PI 1a 2 3  Linearised BAC  probe 9.8 kb 54 kb b c 32 < 16 Tg Line Lo Tg Line Hi 129SvEv C57/bl6 129SvEv 8 Relative Expression (transgenic/wild-type) 8.5 kb < 4 < 7.2 kb 2 < < < < 1 0.5 p16INK4a Arf Krishnamurthy et al., Supp. Figure 2

  3. p16INK4a +/+ p16INK4a TG lung lung cerebellum cerebellum spleen uterus pancreas spleen uterus pancreas Ink4a -/- p16INK4a IP: p16 Krishnamurthy et al., Supp. Figure 3

  4. 40 30 p16INK4a +/+ p16INK4a -/- Weight (grams) 20 10 Age: 17-20 Week 40-52 Week a b 40-52 week old mice (excluding tumor-bearing outliers) 17-20 week old mice 40-52 week old mice (all) 500 500 500 p16INK4a +/+ (N=13) p16INK4a +/+(N=10) p16INK4a +/+(N=10) p16INK4a -/- (N=13) 400 400 400 p16INK4a -/- (N=6) p16INK4a -/-(N=8) 300 Serum glucose (mg/dl) 300 300 200 200 200 100 100 100 0 20 40 60 80 100 0 20 40 60 80 100 0 20 40 60 80 100 Minutes post-IP glucose Krishnamurthy et al., Supp. Figure 4

  5. * Islet Size %Ki67+ islet cells * * Islet area (arbitrary units) ki67 positive * # 12% 10.0 10% Days post-STZ treatment 7.5 8% 5.0 6% 4% 2.5 2% 0 0% 0 4 8 12 16 20 24 28 a b 3 days post-STZ Untreated Krishnamurthy et al., Supp. Figure 5

  6. tonsil appendix Ki67 = brown stain Krishnamurthy et al., Supp. Figure 6

More Related