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Preventing Introduction, Growth and Cross-Contamination of Listeria monocytogenes. ©2006 Department of Food Science - College of Agricultural Sciences at Penn State University Penn State is committed to affirmative action, equal opportunity, and the diversity of its workforce.
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Preventing Introduction, Growth and Cross-Contamination of Listeria monocytogenes ©2006 Department of Food Science - College of Agricultural Sciences at Penn State University Penn State is committed to affirmative action, equal opportunity, and the diversity of its workforce. This publication is available in alternative media on request.
Contamination • Introduction • LM is brought into the facility from an outside source • Growth • LM present in the facility/food has the ability to multiply • Cross-contamination • LM present in the facility/food is transferred to another food or surface
Introduction of LM Non-food items Rodents/Pests Retail Facility Vendors Employees Customers Contaminated Food
Retail establishments are open to the public • Restrictions & controls should be designed to complement normal operations
Introduction of LM • Employees • Sources • Ill employees • Unclean hands, clothing • Prevention • Good personal hygiene • Restriction of ill employees • Training
Introduction of LM • Vendors • Sources • Ill vendors • Unclean hands or clothing • Prevention • Restriction from high risk areas • Training/vendor agreements
Introduction of LM • Customers • Sources • Ill customer • Unclean hands • Prevention • Facilitate proper handing of food • Tongs, single use paper • Instructions for proper handing
Introductions of LM • Rodents/Pests • Sources • Feces • Rodents/pest carrying LM on feet and body • Prevention • Pest control program • Maintenance of indoor and outdoor facilities
Introduction of LM • Contaminated Food • Sources • Raw products (meat, poultry, fruits, vegetable) • Ready-to-eat foods • Prevention • Vendor assurances (HACCP plan, testing) • Vendor audits
Introduction of LM • Non-food Items • Sources • Contaminated packaging, other supplies • Prevention • Receiving standards
Growth of LM • Since it is not possible to eliminate all sources of LM in the retail environment, preventing growth is essential • On Food • On Equipment • In the Environment
Controls to Prevent Growth • Adequate time and temperature controls • Proper cleaning and sanitation • Good personal hygiene
Time/Temperature Controls • Cold Holding (Refrigeration) 41ºF for 7 days OR 45ºF for 4 days • LM can grow at refrigeration temperatures, so holding time is important • Control of LM growth is the basis for 2005 Food Code cold holding temperature/time combinations
Time/Temperature Control • Date Marking • Requires active managerial control • Applies to: • Certain high risk foods • Foods held for greater than 24 hours • After prepared by a food establishment • After opened, if a processed food
Estimated Reduction of Cases of Listeriosis from Limits on Refrigeration Temperatures
Time/Temperature Controls • Hot holding • Minimum of 135ºF • No temperature control • Can be held for no longer than 4 hours at ambient temperatures
Cleaning & Sanitation • Prevention of LM growth requires a sanitation program that is: • Properly designed • Properly carried out • Target areas • Food contact equipment at room temperature • Refrigerated storage & display cases
Controls to Prevent Cross-Contamination • Cleaning & Sanitation • Good Personal Hygiene • Proper Flow of Food & Employees
Cleaning & Sanitation • To prevent cross-contamination • Always clean between working with raw and ready-to-eat products • Regularly clean to reduce chance that contaminated product transfers to uncontaminated product
Personal Hygiene • Handwashing stations • Easily accessible to promote frequent handwashing • Properly supplied • Appropriate training • Employees should understand concept of cross-contamination
Proper Flow of Food • Facility design • Separation of raw and ready-to-eat areas • Adequate storage and work space • Refrigeration • Preparation tables • Display cases
Flow of Employees • Facility design • Location of hand washing facilities • Locations of locker & rest rooms • Separation of raw and ready-to-eat areas • Designated tasks for employees • Designated work areas
Refuse Storage Receiving Locker / Rest Rooms Dishwashing Area Dry Storage Refrigerated Storage Employee Entrance Cooler Prep Table Prep Table Handwashing Sink Service Area / Cooler Customer Entrance Flow of Employees
Prevention of LM • Prevent • Introduction • Growth • Cross-contamination