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Binhua P. Zhou1 et al., Nature Cell Biology , 2004 

Dual regulation of Snail by GSK-3 -mediated phosphorylation in control of epithelial–mesenchymal transition. Binhua P. Zhou1 et al., Nature Cell Biology , 2004 . P. P. GSK3- β. proteasome. Snail. ?. I. II. GSK3- β. Snail. ?. P. P. < Immuno-complex kinase assay >. -Myc. IP : Myc.

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Binhua P. Zhou1 et al., Nature Cell Biology , 2004 

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  1. Dual regulation of Snail by GSK-3 -mediated phosphorylation in control of epithelial–mesenchymal transition Binhua P. Zhou1 et al., Nature Cell Biology , 2004 

  2. P P GSK3-β proteasome Snail ?

  3. I II GSK3-β Snail ?

  4. P P < Immuno-complex kinase assay > -Myc IP: Myc Myc- + r-P32 ATP IP:Flag- GST-snail I GSK3-β Snail

  5. P P P P P P Stability Snail Snail Snail Half-lives Snail Snail Snail

  6. P P P P P Snail Snail Snail Snail Snail Snail

  7. Snail MCF7 Snail (-)

  8. P P P Snail Snail Snail

  9. ② ① Biochim Biophys Acta. 2004

  10. Biochim Biophys Acta. 2004

  11. P P EGF,IGF EGF,IGF Akt, MAPK X Akt, MAPK GSK -3β X GSK -3β Snail Snail E-cad X E-cad

  12. P P EGF,IGF EGF,IGF X Akt, MAPK Akt, MAPK GSK -3β GSK -3β X Snail Snail X E-cad E-cad

  13. * Wnt-dependent regulation of the E-cadherin repressor snail (JBC,2005) : Snail displays ß-catenin-like canonical motifs that support its GSK3ß-dependent phosphorylation, ß-TrCP-directed ubiquitination and proteasomal degradation. : Wnt signaling inhibits Snail phosphorylation and consequently increases Snail protein levels and activity, while driving an in vivo epithelial-mesenchymal transition that is suppressed following Snail knockdown.

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