Cells and Tissues

1. Cells and Tissues

2. Cell Physiology: Membrane Transport • Membrane transport—movement of substances into and out of the cell • Two basic methods of transport • Passive transport • No energy is required • Active transport • Cell must provide metabolic energy (ATP)

3. Solutions and Transport • Solution—homogeneous mixture of two or more components • Solvent—dissolving medium; typically water in the body • Solutes—components in smaller quantities within a solution • Intracellular fluid—nucleoplasm and cytosol • Interstitial fluid—fluid on the exterior of the cell

4. Selective Permeability • The plasma membrane allows some materials to pass while excluding others • This permeability influences movement both into and out of the cell

5. Passive Transport Processes • Diffusion • Particles tend to distribute themselves evenly within a solution • Movement is from high concentration to low concentration, or down a concentration gradient Figure 3.9

6. Passive Transport Processes • Types of diffusion • Simple diffusion • An unassisted process • Solutes are lipid-soluble materials or small enough to pass through membrane pores

7. Passive Transport Processes Figure 3.10a

8. Passive Transport Processes • Types of diffusion (continued) • Osmosis—simple diffusion of water • Highly polar water molecules easily cross the plasma membrane through aquaporins

9. Passive Transport Processes Figure 3.10d

10. Passive Transport Processes • Facilitated diffusion • Substances require a protein carrier for passive transport • Transports lipid-insoluble and large substances

11. Passive Transport Processes Figure 3.10b–c

12. Passive Transport Processes • Filtration • Water and solutes are forced through a membrane by fluid, or hydrostatic pressure • A pressure gradient must exist • Solute-containing fluid is pushed from a high-pressure area to a lower pressure area

13. Active Transport Processes • Substances are transported that are unable to pass by diffusion • Substances may be too large • Substances may not be able to dissolve in the fat core of the membrane • Substances may have to move against a concentration gradient • ATP is used for transport

14. Active Transport Processes • Two common forms of active transport • Active transport (solute pumping) • Vesicular transport • Exocytosis • Endocytosis • Phagocytosis • Pinocytosis

15. Active Transport Processes • Active transport (solute pumping) • Amino acids, some sugars, and ions are transported by protein carriers called solute pumps • ATP energizes protein carriers • In most cases, substances are moved against concentration gradients • http://www.youtube.com/watch?v=awz6lIss3hQ

16. Extracellular fluid Na+ K+ Na+ P Na+ P Na+ Na+ K+ K+ Na+ P K+ ATP ADP Loss of phosphate restoresthe original conformation ofthe pump protein. K+ isreleased to the cytoplasm andNa+ sites are ready to bind Na+again; the cycle repeats. Binding of cytoplasmic Na+to the pump proteinstimulates phosphorylationby ATP, which causes thepump protein to change itsshape. The shape change expelsNa+ to the outside.Extracellular K+ binds,causing release of thephosphate group. Cytoplasm Figure 3.11

17. Extracellular fluid Na+ Na+ Na+ P ATP ADP Binding of cytoplasmic Na+to the pump proteinstimulates phosphorylationby ATP, which causes thepump protein to change itsshape. Cytoplasm Figure 3.11, step 1

18. Extracellular fluid Na+ K+ Na+ P Na+ P Na+ Na+ K+ Na+ P ATP ADP Binding of cytoplasmic Na+to the pump proteinstimulates phosphorylationby ATP, which causes thepump protein to change itsshape. The shape change expelsNa+ to the outside.Extracellular K+ binds,causing release of thephosphate group. Cytoplasm Figure 3.11, step 2

19. Extracellular fluid Na+ K+ Na+ P Na+ P Na+ Na+ K+ K+ Na+ P K+ ATP ADP Loss of phosphate restoresthe original conformation ofthe pump protein. K+ isreleased to the cytoplasm andNa+ sites are ready to bind Na+again; the cycle repeats. Binding of cytoplasmic Na+to the pump proteinstimulates phosphorylationby ATP, which causes thepump protein to change itsshape. The shape change expelsNa+ to the outside.Extracellular K+ binds,causing release of thephosphate group. Cytoplasm Figure 3.11, step 3

20. Active Transport Processes • Vesicular transport • Exocytosis • Moves materials out of the cell • Material is carried in a membranous vesicle • Vesicle migrates to plasma membrane • Vesicle combines with plasma membrane • Material is emptied to the outside

21. Active Transport Processes: Exocytosis Figure 3.12a

22. Active Transport Processes: Exocytosis Figure 3.12b

23. Active Transport Processes • Vesicular transport (continued) • Endocytosis • Extracellular substances are engulfed by being enclosed in a membranous vescicle • Types of endocytosis • Phagocytosis—“cell eating” • Pinocytosis—“cell drinking”

24. Extracellularfluid Extracellularfluid Cytoplasm Plasmamembrane Pit Recycling of membraneand receptors (if present)to plasma membrane Transport to plasmamembrane andexocytosis ofvesicle contents Ingestedsubstance Vesicle Lysosome Detachmentof vesicle Release ofcontents tocytoplasm Vesicle containingingested material Plasmamembrane Vesicle fusingwith lysosomefor digestion (a) Active Transport Processes: Endocytosis Figure 3.13a

25. Extracellularfluid Extracellularfluid Plasmamembrane Cytoplasm Pit Ingestedsubstance Plasmamembrane (a) Active Transport Processes: Endocytosis Figure 3.13a, step 1

26. Extracellularfluid Extracellularfluid Plasmamembrane Cytoplasm Pit Ingestedsubstance Detachmentof vesicle Vesicle containingingested material Plasmamembrane (a) Active Transport Processes: Endocytosis Figure 3.13a, step 2

27. Extracellularfluid Extracellularfluid Plasmamembrane Cytoplasm Pit Ingestedsubstance Vesicle Lysosome Detachmentof vesicle Vesicle containingingested material Plasmamembrane Vesicle fusingwith lysosomefor digestion (a) Active Transport Processes: Endocytosis Figure 3.13a, step 3

28. Extracellularfluid Extracellularfluid Plasmamembrane Cytoplasm Pit Ingestedsubstance Vesicle Lysosome Detachmentof vesicle Release ofcontents tocytoplasm Vesicle containingingested material Plasmamembrane Vesicle fusingwith lysosomefor digestion (a) Active Transport Processes: Endocytosis Figure 3.13a, step 4

29. Extracellularfluid Extracellularfluid Plasmamembrane Cytoplasm Pit Transport to plasmamembrane andexocytosis ofvesicle contents Ingestedsubstance Vesicle Lysosome Detachmentof vesicle Release ofcontents tocytoplasm Vesicle containingingested material Plasmamembrane Vesicle fusingwith lysosomefor digestion (a) Active Transport Processes: Endocytosis Figure 3.13a, step 5

30. Extracellularfluid Extracellularfluid Plasmamembrane Cytoplasm Pit Recycling of membraneand receptors (if present)to plasma membrane Transport to plasmamembrane andexocytosis ofvesicle contents Ingestedsubstance Vesicle Lysosome Detachmentof vesicle Release ofcontents tocytoplasm Vesicle containingingested material Plasmamembrane Vesicle fusingwith lysosomefor digestion (a) Active Transport Processes: Endocytosis Figure 3.13a, step 6

31. Active Transport Processes: Endocytosis Figure 3.13b–c

32. Active Transport Processes