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RNA Components of Spliceosome Active Site

RNA Components of Spliceosome Active Site. Complex RNA Protein Assemblies. S. cerevisiae - 4 RNAs -~80 proteins 66% RNA. H. sapiens -5 RNAs (~400 nt) -80-100 proteins 5% RNA. Complex RNA Protein Assemblies. S. cerevisiae - 4 RNAs -~80 proteins 66% RNA. H. sapiens -5 RNAs (~400 nt)

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RNA Components of Spliceosome Active Site

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  1. RNA Components of Spliceosome Active Site

  2. Complex RNA Protein Assemblies S. cerevisiae - 4 RNAs -~80 proteins 66% RNA H. sapiens -5 RNAs (~400 nt) -80-100 proteins 5% RNA

  3. Complex RNA Protein Assemblies S. cerevisiae - 4 RNAs -~80 proteins 66% RNA H. sapiens -5 RNAs (~400 nt) -80-100 proteins 5% RNA

  4. U5 snRNP (8) U1 snRNP (3) U5-220kD PRP8 U1-70kD SNP1 U5-200kD BRR2 U1 A MUD1 U5-116kD SNU114 U1 C YHC1 U5-102kD PRP6 U5-100kD PRP28 U5-52kD SNU40 U2 snRNP (10) U5-40kD SAP155 HSH155 U5-15kD DIB1 SAP145 CUS1 U4/U6 snRNP (5) SAP130 RSE1 SAP114 PRP21 U4/U6-90kD PRP3 SAP62 PRP11 U4/U6-60kD PRP4 SAP61 PRP9 U4/U6-61kD PRP31 SAP49 HSH49 U4/U6-20kD CPH1 U2 A' LEA1 U4/U6-15.5kD SNU13 U2 B" MSL1 p14 SNU17 U4/U6.U5 tri-snRNP (3) Tri-snRNP 110kD SNU66 Sm/LSm core (33) Tri-snRNP 65kD SAD1 Tri-snRNP 27kD Sm B/B' SMB1 Sm D1 SMD1 Sm D2 SMD2 Sm D3 SMD3 Sm E SME1 Sm F SMX3 Sm G SMX2 hLSm2 LSM2 hLSm3 LSM3 hLSm4 LSM4 hLSm6 LSM6 hLSm7 LSM7 Spliceosome Proteomics (~60 proteins)

  5. Catalytic step II/late (6) Non-snRNP assembly (15) hPrp16 PRP16 SRm300 hPrp22 PRP22 hPrp5 PRP5 hPrp43 PRP43 hCrn CLF1 hSlu7 SLU7 CDC5L CEF1 hPrp17 PRP17 hCBP80 CBP80 hPrp18 PRP18 SF1 BBP SKIP PRP45 PUF60 PLRG1 PRP46 hPrp19 PRP19 U2AF65 MUD2 U2AF35 SPF30 hCBP20 CBP20 hFBP11 PRP40 SR (10) SRp75 p54/SFRS1 SRp55 SRp40 ASF/SF2 9G8 SC35 SRp30c hTra2 SRp20 Spliceosome+ Proteomics (~20-30+ proteins)

  6. Spliceosomal Rearrangements Spliceosomal Rearrangements A GU AG

  7. Spliceosomal Rearrangements Spliceosomal Rearrangements A GU AG

  8. Spliceosomal Rearrangements Spliceosomal Rearrangements BBP U2AF A GU AG

  9. Spliceosomal Rearrangements Spliceosomal Rearrangements A GU AG

  10. A UGCUG C AUGAUG U2 snRNA Spliceosomal Rearrangements Spliceosomal Rearrangements A GU AG

  11. Spliceosomal Rearrangements Spliceosomal Rearrangements A GU AG

  12. Spliceosomal Rearrangements GU AG A

  13. RRM•RNA Recognition Cell98, 835 (1999).

  14. U2AF65•Nucleic Acid Complex Mol Cell. 2006 23 49-59

  15. Branch Recognition • 1 (SF1•ssRNA) Science (2001) 294, 1098-1102

  16. Branch Recognition • 2 (SF1•ssRNA) Proc Natl Acad Sci USA. 2006 103, 1266-71.

  17. Surface Pocket SF3b14•SF3b155

  18. U5 snRNP Component PRP8 3' SS 5' SS 1475 1588 1669 2034 IV 3.2 RRM MPN 1078 1 986 2335 1894 -1898

  19. U5 snRNP Component PRP8 Nat Struct Mol Biol. 2008 15 1199-205.

  20. U5 snRNP Component PRP8

  21. U1 snRNP Nature. 2009 458 475-80.

  22. U1 snRNA

  23. 5’ Splice Site Recognition

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