Acetylcholinesterase inhibitory-active components of Rhodiola rosea L.

1. Acetylcholinesterase inhibitory-active components of Rhodiolarosea L. Hua Wang, Gaochao Zhou, Xiangdong Gao, Yidan Wang, Wenbing Yao * School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia xiang, Nanjing 210009, PR China 授課老師：詹于誼 教授 報告者：田仲源、莫博欽

2. Identification of compound 1Hydroquinone: acicular crystals; UV max at 285 nm;[M-1] peak at m/z 110; 1H NMR (500 MHz, D2O) d6.78 (2H, s), 4.70 (4H, s); 1H NMR (500 MHz, DMSOD6)d 8.57 (2H, s), 6.55 (4H, s); 13C NMR (300 MHz,DMSO-D6) d 149.9 (s, C-1, 4), 115.8 (s, C-2, 3, 5, 6) [identicalwith the Nist database and SDBS database].

3. AChE inhibitory activity of HQ

6. Discussion and conclusion • Alzheimer’s disease (AD) is the most common form of dementia among the elderly. In AD patients, there is a decreased level of acetylcholine in the brain areas related to memory and learning. • the AChE inhibitory-active components chloroform ＞ n-butanol ＞ ethyl acetate ＞water • false-positive effect the sample only inhibits the chemical reaction between thiocholine and DTNB, but not the enzymatic reaction.

7. This purified compound demonstrated AChE inhibitory activity with an IC50 of 9.81 × 103 mg/ml • This not only helped our further understanding of the anti-acetylcholinesterase activity of RR, but also had an implication in the food industry for use as a natural AChE inhibitor and supplement against AD.

8. Thanks for your attention