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A VACCINE FOR WOOL PRODUCTION

A VACCINE FOR WOOL PRODUCTION. CSIRO Livestock Industries Centre for Environment and Life Sciences Perth, WA. There Are 2 Distinct Groups of Rumen Protozoa. Those with reduced cilia, spines, and skeletal plates. Those entirely covered with cilia with no spines and skeletal plates.

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A VACCINE FOR WOOL PRODUCTION

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  1. A VACCINE FOR WOOL PRODUCTION CSIRO Livestock Industries Centre for Environment and Life Sciences Perth, WA

  2. There Are 2 Distinct Groups of Rumen Protozoa Those with reduced cilia, spines, and skeletal plates Those entirely covered with cilia with no spines and skeletal plates

  3. Biology of Rumen Protozoa bacteria largespecies, Isotricha prostoma macronucleus cilia cell throat (cytopharynx) cell mouth (cytostome) smallspecies, Dasytricha ruminantium

  4. Why Manipulate Rumen Protozoa? • protozoa make-up 50-80% microbial biomass • protozoa consume other protozoa and recycle microbial protein in the rumen • removing protozoa can increase the flow of microbial protein to the intestine by 50% • protozoa are a major source of H2 for methane production

  5. Key AWI Project Outcomes • develop a commercial vaccine product that selectively • targets and suppresses rumen protozoa • increase the levels of circulating amino acids available for wool production and growth • benefits could accrue from reduction in methane emissions and nitrogen excretion

  6. Patent Protection We are the leader in this research area and CSIRO already has patents granted in these areas. PATENT NO.COUNTRY TW5684/AU Australia TW5684/NZ New Zealand TW5684/ZA South Africa TW5684/EP European Union TW5684/US USA

  7. Patent Data – Anti-Protozoa Vaccine

  8. Early Results - 2004 • raised antibodies against protozoa in chicken & sheep • completed in vitro testing • of 8 different vaccine • formulations • animal house experiment • currently being conducted • with 1-2 preferred vaccines • next step is field testing

  9. Pre-Immune Control • cells pear-shaped and intact • large contractile vacuole seen

  10. Post-Immunization (Time Point 1 of 4) • cell membrane compromised • cell losing normal pear-shaped appearance • cell on right beginning to lose its cytoplasm

  11. Post-Immunization (Time Point 2 of 4) • now both cells are seen losing their cytoplasm

  12. Post-Immunization (Time Point 3 of 4) • small cell in the centre now losing its cytoplasm

  13. Post-Immunization (Time Point 4 of 4) • both large cells have been destroyed

  14. Brightness indicates binding of antibodies to rumen protozoa x320 x320 • incubated with • post-immune plasma • incubated with • pre-immune plasma

  15. The Research Team Andrew Williams Suzy Rea Andre Wright Sam Popovski Carolyn Pimm Louis Klein Andrew Toovey Yvette Williams Lucy Skillman Roslyn Owen

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