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Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai

Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai. Novel nanobiosensors. Simple, fast and cost -effective tools for biodiagnostics and medical research. Technologies originating from the research results of Prof . Denis Boudreau (COPL, Université Laval).

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Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai

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  1. Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai

  2. Novel nanobiosensors Simple, fast and cost-effective toolsfor biodiagnosticsand medicalresearch Technologies originating from the research results of Prof. Denis Boudreau (COPL, Université Laval) Presented by Martin Castonguay, SOVAR Parlons Affaires Photonics North 2014, Montréal, 29 mai

  3. Technology: “what and why ?”Plasmonic fluorescent core-shell nanoparticles Silver core for enhanced fluorescence Silvercore (~50 nm dia.) Strong signal Resistance to photobleaching Excellent signal/noise Target molecule captured by molecularprobe (strong signal) Molecular probe for specificity Silica surface for easy immobilization of biomolecules Molecular probe (no signal) Silica shell (~3 nm th.) Versatility Parlons Affaires Photonics North 2014, Montréal, 29 mai

  4. Application example #1: PCR-free genotyping • Phenotyping and immunological methods are gradually being replaced by genotyping methods • Genotyping methods utilizing PCR amplification of genetic material are costly, complex and time-consuming • PCR-free cost-effective and timely genotyping tools would increase time-to-answer and decrease complexity and costs Parlons Affaires Photonics North 2014, Montréal, 29 mai

  5. Technological advantagesDNA sensing based on fluorescent nanoparticles and a polymeric hybridization transducer • Sensitive:1000 copies, vs. 10 copies for PCR • ~10M copies are available from 1 ml of blood • Rapid: result available <10 min after mixing • Simple sample preparation AND no amplification required • Low cost:straightforward fabrication process and readily available instrumentation for detection Parlons Affaires Photonics North 2014, Montréal, 29 mai

  6. Proof of principleSRY and Dombrock genesweredetecteddirectlyfrom DNA extractedfromhumanbloodcells Brouard D, Ratelle O et al. (2013) Analytical Methods 5(24) Unpublished data (2014). Fluorescence signal (a.u.) Fluorescence signal (a.u.) SRY genotyping results for 10 non-amplified genomic DNA samples.1 Dob (-) Dob (+) Doa (-) Doa (+) Female donors Male donors Dombrock (Doa/Dob) genotyping results for 6 non-amplified genomic DNA samples.2 Parlons Affaires Photonics North 2014, Montréal, 29 mai

  7. Preliminary evaluation of the cost per test Parlons Affaires Photonics North 2014, Montréal, 29 mai

  8. Application example #2: Functional cell imaging • Stable grafting on surfaces • Non-invasive measurements • Quantitative invitro analyses • Multi-elementaryresponse • Commercial microscopyhardware • Adaptability to differentfunctionalfluorophores • pH sensibility • Calcium imagery • Chloride • Sodium, Potassium • DNA probes “Fluorescent nanosensors and uses thereof”, Canada/US provisional patent filed May 23, 2014. Parlons Affaires Photonics North 2014, Montréal, 29 mai

  9. Substrate fluorescence Proof of principleLive extracellular pH measurements on calcifiedcardiacfibroblastsdirectly in the culture medium pH = 6.5 Addition of therapeuticdrug • Adaptable for variousstudies • Heart and lungcells (Na+, K+, pH) • Cancer and tumors (pH, Cl-, Ca2+) • Neurons (Ca2+, neurotransmitters) Parlons Affaires Photonics North 2014, Montréal, 29 mai

  10. IP protection • Patent 1: Polymer transducer • Canada, US, Europe (UK, France, Germany), Australia and Japan • Patent 2: Combination of polymer transducer to fluorescent reporter • Canada, US, Europe (UK, France, Germany, Spain, Swiss), Australia and Japan • Patent 3: Combination of polymer transducer and particles • Canada, US. • A patent application was just filed a week ago for the surface-grafted nanoparticles. Parlons Affaires Photonics North 2014, Montréal, 29 mai

  11. Take home message ? Prof. Boudreau and his team are experts in the field of nanoparticles. If you are interested in functional nanoparticles for medical or other applications, there may well be a match between his science and your business needs ! Fast time-to-answer Low-cost adaptable product Benefits for Canadians International market Uses existingdetection hardware denis.boudreau@chm.ulaval.ca Multiple applications Pathogendetection Newborn screening Cancer Pharmacogenomics Parlons Affaires Photonics North 2014, Montréal, 29 mai

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