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1. Title: Physiological and Proteomic Alterations in Rice (Oryza sativa L.) Seedlings under Hexavalent Chromium Stress Journal: Planta Authors: Fanrong Zeng · Xiaojian Wu · Boyin Qiu · Feibo Wu · Lixi Jiang · Guoping Zhang* Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China * Corresponding author. E-mail address: zhanggp@zju.edu.cn (G. Zhang).

2. 10 pI 3 Mr (kDa) 174.6 66.2 45.0 31.0 21.5 14.4 Control 2 µM Cr6+ 200 µM Cr6+ b c a Xiushui113 f d e DanK5 Supplementary Fig. S1 Two-dimensional electrophoresis (2-DE) gel maps of total leaf proteome of rice seedlings in Xiushui113 (A-C) and DanK5 (D-E) grown under control (A and D), 2 µM Cr6+ (B and E) and 200 µM Cr6+ (C and F) treatments. Chromium treatments were initiated when seedlings were 15 d old. Total leaf protein was isolated from the plants subjected to Chromium treatments for 3 days. A sum of 150 µg of protein was loaded on a pH 3 – 10 IpG strip and protein spots were visualized by silver staining. Mr, relative molecular weight; pI, isoelectric point.