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Enzymes used in pyrosequencing 1) DNA polymerase 2) ATP sulfurylase (sulfur metabolism in microorganisms) adenosine-5’-p

Enzymes used in pyrosequencing 1) DNA polymerase 2) ATP sulfurylase (sulfur metabolism in microorganisms) adenosine-5’-phosphosulfate + PP i  ATP + sulfate 3) Luciferase (fireflies) luciferin + ATP + O 2  oxyluciferin + AMP + PP i + CO 2 + light 4) apyrase ATP + H 2 O  AMP + PP i.

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Enzymes used in pyrosequencing 1) DNA polymerase 2) ATP sulfurylase (sulfur metabolism in microorganisms) adenosine-5’-p

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  1. Enzymes used in pyrosequencing 1) DNA polymerase 2) ATP sulfurylase (sulfur metabolism in microorganisms) adenosine-5’-phosphosulfate + PPi ATP + sulfate 3) Luciferase (fireflies) luciferin + ATP + O2 oxyluciferin + AMP + PPi + CO2 + light 4) apyrase ATP + H2O AMP + PPi

  2. Parallel sequencing on the Biotage 454 sequencer

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