R. Massiliae plasmid

Nov 02, 2014

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R. massiliae plasmid. R. prowazekii chromosome. R. felis plasmid. R. felis plasmid. R. prowazekii chromosome. R. Massiliae plasmid. Figure S11.

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R. Massiliae plasmid

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R. massiliae plasmid R. prowazekii chromosome R. felis plasmid R. felis plasmid R. prowazekii chromosome R. Massiliae plasmid Figure S11

Modeling Plasmid Selection

Modeling Plasmid Selection Joy Killough RET Teacher University of Texas at Austin With Dr. C. Randall Linder Environmental Science Institute Dr. Jay Banner, Director A Research Experience for Teachers Lesson Program Funding from National Science Foundation Modeling Plasmid Selection

652 views • 16 slides

Plasmid Isolation

590 views • 17 slides

Plasmid Isolation

Overall Picture. Plasmid IsolationRemove plasmid pBS 60.6 from DHa E. coli. Restriction Enzymes. Each restriction enzyme cuts at different sites The cut can leave either blunt or sticky endsIf the plasmid and gene to be inserted into the plasmid are cut with the same restriction enzyme, the sticky ends have overlapping base pairs and can anneal with DNA ligaseBut, the cut ends can anneal to themselves or the cut vector can reanneal to itself Can prevent vector religation by using two restri21

1.18k views • 14 slides

Plasmid ohne Resistenzgenen

Transformation. Grundlagen:. Ampicillin. Tetracyclin. Plasmid ohne Resistenzgenen. Plasmid mit Resistenzgenen. Bakterien besitzen die Fähigkeit Antibiotika In ihrer Wirkung abzuschwächen oder total

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Lab 2a Restriction Digestion of Plasmid DNA ( pARA-R)

Lab 2a Restriction Digestion of Plasmid DNA ( pARA-R). Lab 2. Pre Lab Readiness . Familiarity and proper use of micropipettes Remember the 1 st and 2 nd stops Understand enzymes Not consumed in reaction Affected by time, pH, temp Biological catalyst made of protein

528 views • 9 slides

pGLO plasmid

pGLO plasmid. Beta Lactamase Ampicillin resistance Green Fluorescent Protein (GFP) Aequorea victoria jellyfish gene araC regulator protein Regulates GFP transcription. ara Operon. lac Operon. araC. B. A. D. LacI. Z. Y. A. Effector (Arabinose). Effector (Lactose). araC. B. A.

2.18k views • 7 slides

Plasmid

Plasmid. Plasmid vector?. Plasmid.

1.21k views • 13 slides

Modeling Plasmid Selection

Modeling Plasmid Selection. And Authentic Assessment. 6-2010. http://biology2020.wikispaces.com/. With the pGlo Plasmid. Bacteria are very useful organisms in genetic engineering. They are able to bring in plasmids. Into which DNA of interest may be added. The Problem?.

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Transfected plasmid

Figure S5. BJAB-LMP1 Luc. Transfected plasmid.

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Mystery Plasmid B ??????

Mystery Plasmid B ??????. Preliminary Work. 18 Possible Plasmids 11 Available Enzymes Too much work… Simplify: 3 Digestions. First Set of Digestions. AseI, BamHI Double Digestion DNA 3 μ L (1000ng/ μ L) AseI .75 μ L BamHI .75 μ L BSA 1.5 μ L Buffer 3 1.5 μ L H 2 0 7.5 μ L

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Plasmid Minipreps

Plasmid Minipreps. Kits…. Mini-Prep:. A rapid, small scale method of obtaining or retrieving plasmid DNA (plasmid DNA + foreign/inserted DNA) from bacterial cells. 2 General Variations: 1. Boiling Method 2. Alkaline Lysis Method (With a modification to collect plasmids). Overall Goals:.

967 views • 13 slides

geschnittenes Plasmid

Herstellung eines transgenen Bakteriums. Das Humaninsulin-Gen wird isoliert und mit dem entsprechenden Restriktionsenzym geschnitten. Es enthält nun die gleichen sticky-ends wie das geschnittene Plasmid. Plasmid mit Ampicillin-Resistenz-Gen und ß-Galaktosidase-Gen.

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Plasmid maintenance

Plasmid maintenance. Maintaining plasmids and expressing those genes is energetically expensive to a bacterial cell. It is beneficial only if the bacteria obtains something useful from doing so.

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Plasmid Miniprep

Plasmid Miniprep. Broad and Long Term Objective. To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis. Research Plan. Preparation of Competent Cells/Bacterial Transformation. Growth of Transformant and Plasmid MiniPrep. DNA Sequencing.

317 views • 16 slides

Plasmid DNA Isolation

Plasmid DNA Isolation. Exercise 8. Experiment Goals. Extraction of plasmid DNA from E. Coli Analyze plasmid DNA by agarose gel electrophoresis and spectrophotometer. Plasmid. Plasmids.

1.86k views • 22 slides

Plasmid purification lab

Plasmid purification lab. Plasmid. What is the plasmid ? Plasmid are small, double strand , closed circular DNA molecules , that can be isolated from bacterial cells , they replicate independently of the bacterial chromosome. Features. 1. Found in a wild variety of bacterial species.

439 views • 13 slides

Typical Plasmid

Typical Plasmid. Blue/White Selection. Alpha complementation Trick. omega. alpha. Blue/White Selection. Standard 4 exon gene. -Where is the polyA tail in the gene above? -Draw a 4 exon gene where exon 4 is all 3’UTR. Chapter 11 Transcription and RNA Processing. The Central Dogma.

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PLASMID RECORD

PLASMID RECORD. Name: ..................................................................................................... Date: ..................... Size: ....................................... Vector: ..................................................................................

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Plasmid mediated Metallo-Beta-Lactamase (MBL) Plasmid mediated

Plasmid mediated Metallo-Beta-Lactamase (MBL) Plasmid mediated Hydrolyses all beta-lactam (except aztreonam) Inhibited by EDTA Binds zinc (Zn 2+ ) Pseudomonas aeruginosa or Enterobacteriaceae.

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Plasmid 24307 7TFC

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 24307 with the following enzymes: PstI. Plasmid 24307 7TFC. PstI. Uncut. 1kb Ladder. 1.0 1.2 1.5 2 3 4 5 6 8 10.

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whole plasmid sequenicng

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plasmid dna sequencing

CD Genomics provides complete plasmid DNA sequencing service, and our improved bioinformatics pipelines are available to perform de novo assembly with no reference required.

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