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Supplementary Figure 3 a-d

Supplementary Figure 3 a-d.

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Supplementary Figure 3 a-d

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  1. Supplementary Figure 3 a-d Supplementary Figure 3. Ki16425 is not toxic against RMS cells. Panel A - Proliferation of RH30 cells pretreated for 1h with 10 μM or 50 μM Ki15425 in comparison to control cells pretreated with vehicle only. Panel B – Representative FACS analysis of Anexin V binding and propidium iodide incorporation into RMS cells 24h after pre-treatment with Ki16425. Panel C – Analysis of Anexin V and propidium iodide staining in cells 24h and 48h after 1h pre-treatment with Ki16425. Pre-treatment was done in PBS, than cells were washed out from inhibitor, resuspendedin medium supplemented with 10% FBS and cultured in suspension in low cell adherence dishes. Panel D - Analysis of Anexin V and propidium iodide staining in RMS cells exposed for 48 hours to Ki16425. Cells were incubated in the presence of inhibitor in medium supplemented with 0.5% BSA or 10% FBS.

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