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Evaluation of Ki16425 Toxicity on RMS Cells: Proliferation and Apoptosis Analysis

Study assessing Ki16425 toxicity on RMS cells through proliferation and apoptosis evaluation using FACS analysis at different time points and conditions. The results show no significant toxicity of Ki16425 on the cells.

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Evaluation of Ki16425 Toxicity on RMS Cells: Proliferation and Apoptosis Analysis

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  1. Supplementary Figure 3 a-d Supplementary Figure 3. Ki16425 is not toxic against RMS cells. Panel A - Proliferation of RH30 cells pretreated for 1h with 10 μM or 50 μM Ki15425 in comparison to control cells pretreated with vehicle only. Panel B – Representative FACS analysis of Anexin V binding and propidium iodide incorporation into RMS cells 24h after pre-treatment with Ki16425. Panel C – Analysis of Anexin V and propidium iodide staining in cells 24h and 48h after 1h pre-treatment with Ki16425. Pre-treatment was done in PBS, than cells were washed out from inhibitor, resuspendedin medium supplemented with 10% FBS and cultured in suspension in low cell adherence dishes. Panel D - Analysis of Anexin V and propidium iodide staining in RMS cells exposed for 48 hours to Ki16425. Cells were incubated in the presence of inhibitor in medium supplemented with 0.5% BSA or 10% FBS.

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