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Naoko Yoshida , et al. 2004 Science of the Total Environment 381:233 – 242

Polyphasic characterization of a PCP-to-phenol dechlorinating microbial community enriched from paddy soil. Naoko Yoshida , et al. 2004 Science of the Total Environment 381:233 – 242. Pentachlorophenol (PCP) . Formula: C 6 HCl 5 O insecticide herbicide wood preservative

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Naoko Yoshida , et al. 2004 Science of the Total Environment 381:233 – 242

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  1. Polyphasic characterization of a PCP-to-phenol dechlorinating microbial community enriched from paddy soil Naoko Yoshida , et al. 2004 Science of the Total Environment 381:233–242

  2. Pentachlorophenol (PCP) • Formula: C6HCl5O • insecticide • herbicide • wood preservative • moderately toxic compound in EPA toxicity class II

  3. Toxicological Effects • Acute toxicity • profuse sweating, dehydration, nausea, coma. • Chronic toxicity • obstruction of the circulatory system in the lungs and cause heart failure • Teratogenic effects • PCP has teratogenic effects in humans at normal exposure levels • Organ toxicity • liver, kidneys, and central nervous system http://extoxnet.orst.edu/

  4. Environmental Fate • Breakdown in soil and groundwater • rapidly in flooded or anaerobic soils, anaerobic biodegradation. • half-life 45 days (soil environment). • Breakdown in water • sediments and suspended particles in water. • Surface. • Breakdown in vegetation • Uptake and accumulation varies according to plant species. • PCP is strongly toxic to plants . http://extoxnet.orst.edu/

  5. Anaerobic Microbial Degradation of Haloaromatic Compounds H.Sahm et al.

  6. Goal • Construct the dechlorination pathway of PCP in paddy soil. • Find microbial community associated with the PCP dechlorination in paddy soil.

  7. PCP degradation activities of the transferred microcosm

  8. Possible pathway of PNP dechlorination The preferable dechlorination is ortho- para- meta- position

  9. Effect of Electron Donors Effect of Electron Acceptors Effect of Inhibitory Treatments Gram-postive, spore-forming, classified in Firmicute

  10. Respiratory Quinones in enrich microcosm Respiring bacteria:1.2×109 cells g-1 1.2% of total microbial population

  11. DGGE fragment patterns Archaea Bacteria

  12. Gram-postive, spore-forming, classified in Firmicute

  13. Conclusion • The microcosm can dechlorinate of PCP to Phenol. • A novel anaerobic Gram-postive spore-forming bacteria in PCP dechlorination.

  14. Reference • H.Sahm, M.Brunner and S.M.Schoberth. 1986. Anaerobic Degradation of Halogenated Aromatic Compounds. Microbial Ecology. 12:147-153. • Brett Fisher. 1991. Pentachlorophenol: Toxicology and Environmental Fate. JOURNAL OF PESTICIDE REFORM. VOL. 11, NO. 1 • http://extoxnet.orst.edu/

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