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PGP expression assessed by flow cytometry. CCRF-CEM and VinR (Vincristine resistant) cells, HT29 and WI38. CCRF-CEM VinR1 m g/ml 20.4 Mean fluorescence = 11.5. CCRF-CEM VinR 1 m g/ml 27.4 Mean fluorescence = 15.3. CCRF-CEM Mean fluorescence = 2.5. PMX 500 – PGP expression. HT29
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PGP expression assessed by flow cytometry. • CCRF-CEM and VinR (Vincristine resistant) cells, HT29 and WI38. CCRF-CEM VinR1mg/ml 20.4 Mean fluorescence = 11.5 CCRF-CEM VinR 1mg/ml 27.4 Mean fluorescence = 15.3 CCRF-CEM Mean fluorescence = 2.5 PMX 500 – PGP expression HT29 Mean fluorescence = 1.6 WI38 Mean fluorescence = 2.2 secondary antibody only PGP antibody + secondary antibody