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Enzymes A review of how they work. Generate a definition of Enzymes. Proteins that promote specific rxns in cells by lowering activation energy required to start a rxn. How do enzymes work?. Lower activation energy increase speed of metabolic rates Break down….build up???.
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Generate a definition of Enzymes • Proteins that promote specific rxns in cells by lowering activation energy required to start a rxn.
How do enzymes work? • Lower activation energy • increase speed of metabolic rates • Break down….build up???
Induced fit model • Enzymes are specific • http://www.phschool.com/science/biology_place/labbench/lab2/induced.html • http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_enzymes_work.html
What happens after an enzyme does its job? • Required in small amounts because as they work, not consumed.
Factors that affect how enzymes work? • Temperature • pH • Amount of substrate available
Brainstorm of Enzymes • Saliva • Stomach • Nervous system: AcHase • Milk products • Papaya fruit • Unprocessed fruits • Kiwi: actiniclin • Soybeans (lipoxygenase) • Fireflies (luciferase) • Pineapples (bromelain) • Potatoes • Egg whites • Turnips (peroxidase) • Tears • Yeast (sucrase) • Lactase • Onions • detergents
How do you know “enzymes” are working? http://www.enetez.com/images/default/for_sale.jpg
Challenge • Design an experiment that will test the effectiveness of an enzyme on the protein gelatin. • Stipulations • Experiment will be performed in class period • conduction is totally designed by experimenters • Enzyme will be proteases (source: detergent) • Data collection must be qualitative and quantitative • Review scoring guide for how you will be graded
materials • Test tubes • Petri dishes • gelatin • Water • Detergent (u supply) • Ruler • Straws • Sharpie/wax pens
Exit slip • List of materials • Independent variable • Dependent variable • Constants • Control • Experimental (treatment group) • What are you measuring
http://www2.visalia.k12.ca.us/eldiamante/science/biology/taters/enzyme_mc.htmhttp://www2.visalia.k12.ca.us/eldiamante/science/biology/taters/enzyme_mc.htm