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Speakers : 黄渊华 (HUANG Yuanhua ) 古梦婷 (GU Mengting ) 方荣欣 (FANG Rongxin )

Speakers : 黄渊华 (HUANG Yuanhua ) 古梦婷 (GU Mengting ) 方荣欣 (FANG Rongxin ). What is DNA methylation?. A biochemical process: an addition of methyl group to cytosine or adenine . Stably alters the genes expression as cells divide and differentiate.

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Speakers : 黄渊华 (HUANG Yuanhua ) 古梦婷 (GU Mengting ) 方荣欣 (FANG Rongxin )

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  1. Speakers: 黄渊华 (HUANG Yuanhua)古梦婷 (GU Mengting)方荣欣 (FANG Rongxin)

  2. What is DNA methylation? • A biochemical process: an addition of methyl group to cytosine or adenine. • Stably alters the genes expressionas cells divide and differentiate. • In adult somatic cells, DNA methylation typically occurs in a CpG (CG) dinucleotide context; • non-CpGmethylation is prevalent in embryonicstem cells. DNA methylation from Wikipedia www.web-books.com

  3. What are CpG site and CpG island? • CpG sites or CG sites: a cytosine nucleotide occurs next to a guanine nucleotide in DNA sequence. • CpG islands or CG islands (CGI): genomic regions that contain a high frequency of CpG sites. www.mad-cow.org/exon2

  4. How to detect DNA methylation? • whole-genome bisulphite sequencing (BisSeq) • Highly parallelsequencing in the article. Blue Nucleotides are unmethylatedcytosinesconverted to uracils by bisulfite, Red nucleotides are 5-methylcytosines resistantto conversion. Bisulfite sequencingfrom Wikipedia

  5. Mouse methylome • Using Bis-Seq to obtain base-pair-resolution methylomes of mouse from embryonic stem (ES) cells and neuronal progenitors (NP). • 77.3 billion mappable methylome bases by High-coverage Illumina sequencing.

  6. Features of the mouse methylome Bimodal distribution Hidden Markov Model for classifying DNA methylation regions: fully methylated regions (FMRs) unmethylated regions (UMRs) low-methylated regions (LMRs) • LMRs are generally not CpG islands: • Lower CpG content; • Shorter; • Further reside distal to transcriptional start sites(TSS). Distribution: not randomly but cluster locally

  7. Features of the mouse methylome Composite profile of CpGmethylation Distribution of the regions throughout the genome. A small percentage of LMRs overlap with CpG islands

  8. A ES-specific NP-specific

  9. Why?

  10. First

  11. Interesting

  12. Novel

  13. Different

  14. Solid

  15. First Interesting Novel Different Solid Finds

  16. Thanks

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