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Study of 7chemotypes of thymes. Thymes: Linalool,thuyanol,carvacrol, thymol, lemony,wild,geraniol, borneol. POLYPHENOLS. Coumarins. Aesculus hippocastanum. Ethyl acetate /chloroform 60/40 v/v on silicagel. Flavonoids. Ethyl acetate /chloroform 40:60 v/v on silicagel. Anthocyanins.
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Study of 7chemotypes of thymes Thymes: Linalool,thuyanol,carvacrol, thymol, lemony,wild,geraniol, borneol.
Coumarins • Aesculus hippocastanum Ethyl acetate /chloroform 60/40 v/v on silicagel
Flavonoids Ethyl acetate /chloroform 40:60 v/v on silicagel
Anthocyanins • Malva sylvestris Ethyl acetate MEK formic acid hydrochloric acid 65/10/6/9 v/v on silicagel
Oplc semi preparative mode • ability to perform high performance separations of difficult samples with little to no sample preparation • ability to visually check directly on the chromatographic phase that all compounds have actually been eluted • abiity for rapid scale-up on the same instrument • ability collect several tenth of mg of purecompounds • ability to select either on-line or off-line injection depending on the sample matrix
Oplc semipreparative mode 2 • Personal works • isolation of • cannabidiol from hemp • strychnine, brucine from strychnos • lupanine from lupinus • morphine, codeine, thebaine, papaverine, noscapine from opium