1 / 20

Odile Huchette, research engineer Christèle Bellamy, research technician

G&H February 2003, Liverpool WP3 / cultivation, P4 P4 : persons involved. Odile Huchette, research engineer Christèle Bellamy, research technician Didier Perrin and Eric Vade, research assistants. Influence of environmental and genetic factors on the alliin content of garlic bulbs.

lester
Download Presentation

Odile Huchette, research engineer Christèle Bellamy, research technician

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. G&H February 2003, LiverpoolWP3 / cultivation, P4 P4 : persons involved • Odile Huchette, research engineer • Christèle Bellamy, research technician • Didier Perrin and Eric Vade, research assistants Influence of environmental and genetic factors on the alliin content of garlic bulbs COOPD'OR R&D

  2. G&H February 2003, LiverpoolWP3 / cultivation, P4

  3. G&H February 2003, LiverpoolWP3 / cultivation, P4 Year 3 : what was done • Greenhouse experiment in various S regimes (P4) • Sampling of fresh material during the cultivation treatment for CSO analysis ( P5) • Freeze drying of samples (P4) for total sulfur analysis (P2) • Micro-propagation of 4 genotypes for new experiments in vitro (P4) • Experiment III (October 2002 => April 2003) : Differential display of clones exposed in vitro to various light conditions (intensity + quality) and S nutrition treatments(0 S in macro-nutrients, normal and excess) • Experiment IV (January 2003 => July 2003) : Differential display of clones exposed in vitro to various mineral nutrition treatments(S combined with N) COOPD'OR R&D

  4. G&H February 2003, LiverpoolWP3 / cultivation, P4 Greenhouse conditions 2001 PRI Greenhouse conditions 2002 Plantation in boxes with washed river sand Mineral nutrition with drop irrigation

  5. G&H February 2003, LiverpoolWP3 / cultivation, P4 Greenhouse experiment 2001 COOPD'OR R&D

  6. G&H February 2003, LiverpoolWP3 / cultivation, P4 Greenhouse experiment 2002  3 varieties : PRI, MOR, MES  3 Sulphur nutritiontreatments: 0 (in the macro-elements) = Treatment S- 2 meq (normal) = Treatment TS 8 meq = Treament S++ • Weekly observations :Growing of the leaves and bulbs • Measurements at harvest : -Weight of the bulbs - alliin content (P5) - total sulfur (P2) in progress COOPD'OR R&D

  7. G&H February 2003, LiverpoolWP3 / cultivation, P4 No differences during the growing season between the 3 treatments 01/03 : Plantation 17/06 : Harvest of MES 10-11/07 : Harvest of MOR 16-17/07 : Harvest of PRI Bulb growing = diameter of the bulb / diameter of the neck

  8. G&H February 2003, LiverpoolWP3 / cultivation, P4 Results at harvest  influence of the variety  no significative influence of the S treatment, except for MES COOPD'OR R&D

  9. G&H February 2003, LiverpoolWP3 / cultivation, P4 Conclusion of greenhouse experiments • Alliin contents in bulbs are 3 to 4 times higher in 2002 compared to 2001 • Printanor appeared in 2002 as the richest variety in alliin, contrary to the previous results • Garlic plants are not affected by an absence of sulfur in the macro-nutrients • absorption of S atmospheric to be confirmed in vitro in 2003 • Garlic plants are not significatively affected by the sulfur nutrition alone, except for one variety tested • influence of the nitrogen to be confirmed in vitro and in greenhouse in 2003 COOPD'OR R&D

  10. G&H February 2003, LiverpoolWP3 / cultivation, P4 In vitro experiments 2001/02 • 4 genotypes exposed to various environmental factors... • quality of the light • sucrose concentration of the medium • temperature • cold induction period • ... and various mineral nutritions • sulfur COOPD'OR R&D

  11. G&H February 2003, LiverpoolWP3 / cultivation, P4 Main results of in vitro experiments • Among all the conditions tested, the better to induce more alliin in garlic bulbs : • Sucrose in the medium : 60g/l [0 - 60 - 120 g/l tested] • Cold induction period (2 to 3 months) • Environmental factor : F+i light • Sulphur in the medium : 6 Meq SO3 [0 - 3 - 6 meq tested] • To be confirmed in 2003 : • Influence of the intensity of the light • Effect of higher dosis of sulphur (9 meq) • Effect of nitrogen combined to sulphur • Role of S atmospheric COOPD'OR R&D

  12. G&H February 2003, LiverpoolWP3 / cultivation, P4 Year 4 : what is planed COOPD'OR R&D

  13. 3 varieties : PRI + MES +MORl Gradient in SO4 0 3 9 (in macro-elements) meq 3 mediums (S- TS S++) 22-24°C - P = 16 hrs - F 1 month Cold induction 3°C - P = 10 hrs - F 2 months 22-24°C – P=16 hrs F 950 lux 22-24°C – P=16 hrs F+i - 950 lux 22-24°C – P=16 hrs F 2000 lux G&H February 2003, LiverpoolWP3 / cultivation, P4 In vitro experiment III-2003 • Effect of various light conditions (intensity + quality) and S nutrition

  14. varieties : PRI + MES + MORl + MORd Gradient in SO4 and nitrogen SO4 0 3 9 meq (in macro-elements) Nitrogen N 3N N 2N 3N N/2 N 2N 3N 9 medias 9 22-24°C - P = 16 hrs - F 1 month Cold induction 2 months 3°C - P = 10 hrs - F 22-24°C – P=16 hrs F+i - 950 lux G&H February 2003, LiverpoolWP3 / cultivation, P4 In vitro experiment IV -2003 • Effect of S nutrition combined to N nutrition

  15. G&H February 2003, LiverpoolWP3 / cultivation, P4 In vitro experiments 2003  Observations : - stage of the plant (1= growing / 2=beginning of bulbification / 3=maturation / 4=mature bulb) - number of green leaves - height of the leaves  Measurements at harvest : -weight, size and observation of the bulbs - height of the leaves - alliin content (P5) COOPD'OR R&D

  16. G&H February 2003, LiverpoolWP3 / cultivation, P4 In vitro experiment III-2003 Observation of the 03 February PRI F - 22-24°C P = 16h S- TS S++ COOPD'OR R&D

  17. G&H February 2003, LiverpoolWP3 / cultivation, P4 Greenhouse experiment 2003 (I) Effect of N combined to an excess of S (II) Cloves from experiment 2002 • Measurements : • growing of the leaves (I-II) and bulbs (I) • weight and CSO content of the bulbs (P5) at harvest (I-II) • 3 varieties and 3 treatments : • S++ N = excess of sulfur, N • S++ 2N = excess of sulfur, 2xN • S++ 3N = excess of sulfur, 3xN COOPD'OR R&D

  18. G&H February 2003, LiverpoolWP3 / cultivation, P4 Effect of virus contamination on AlCSOs in garlic bulbs COOPD'OR R&D

  19. G&H February 2003, LiverpoolWP3 / cultivation, P4Deliverables P4 • DP 2 Chinese garlic powder to be analysed • DP 6 Garlic powder issued from various S-fertilisation in field condition • DP 14 Chemical distinction between garlic accessions when cultivated in various sulphur nutrition regimes and under distinct temperatures in vitro • DP 15 Physiological (growth traits) and chemical distinction (CSO) between accessions when cultivated in various sulphur nutrition regimes over two years • DP 19 Chemical distinction (CSO) between accessions when cultivated in various sulphur (greenhouse) and mineral nutrition regimes (in vitro) • DP 22 Paper on the interaction genotype x environment on the sulphur content of garlic plants grown in vitro , in greenhouse and in field conditions • DP 30 Paper on the effect of combined sulphur, nitrogen and selenium nutrition on garlic growth, flavour precursor content and on biological potential of garlic for disease prevention • DP 34  Chemical distinction (CSO) between garlic accessions when cultivated in various sulphur regimes (greenhouse) and mineral nutrition regimes (in vitro)

  20. G&H February 2003, LiverpoolWP3 / cultivation, P4 garlic powder available from the experiment 2002 in greenhouse 2 varieties :Morasol, Printanor 3 treatments : • S- : no sulphur in the macro-nutrients • TS : 2 meq S in the fertilising solution • S++ : 8 meq S in the fertilising solution COOPD'OR R&D

More Related