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BIO 201 Lab 13 Exp. 16: Week 3 Unknowns

Learn how to identify unknown microorganisms in lab experiment 16. Detailed steps, tips, and grading criteria provided. Good luck!

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BIO 201 Lab 13 Exp. 16: Week 3 Unknowns

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  1. BIO 201 Lab 13Exp. 16: Week 3 Unknowns Professor Diane Hilker GOOD LUCK!

  2. Overview • Exp. 16: Identification of Unknown Microorganisms

  3. I. Exp. 16: Identification of Unknown Microorganisms • TODAY: • Get out all plates, tubes and slides from the incubators that belong to you • Interpret results carefully • Gram Pos. Cocci: Observe Salt Plate for heavy growth; repeat Catalase Test • Gram Pos. Rods: Add IKI to Starch Plate & observe for clearing surrounding the colony which would indicate starch hydrolysis

  4. I. Exp. 16: Identification of Unknown Microorganisms • Gram Negative Rods (Refer to Exp. 21, Table 9 and Figure 8): • Observe PRLB & PRDB tubes first • If both neg., think about Pseudomonas • Split MRVP tube and do the MR Test in one tube and the VP Test in the second tube (may require up to 60 minutes of incubation for color change) • Add chemicals to the necessary tubes • Use Reference Books and uninoculated tubes to assist with your interpretation

  5. I. Exp. 16: Identification of Unknown Microorganisms • Gram Negative Rods: • Interpret YOUR results first & then compare to Table 9 • Use a “Tally or Check Method” • If your results agrees with a microbe give the microbe a check mark • May want to use half checks for +/- and -/+ results • Should be more checks next to one microbe versus the others

  6. I. Exp. 16: Identification of Unknown Microorganisms • When ready to identify your unknown, present to you instructor the following: • Unknown worksheet filled out with your results & Genus identification • Have all tubes, plates and slides available for review • Be able to explain to your instructor your results &what led you to your identification • Instructor will let you know if you are correct in your identification

  7. I. Exp. 16: Identification of Unknown Microorganisms • The BIO201 lab counts 25% of your TOTAL grade • The unknown counts 10% of the BIO201 Lab grade • If you identify your unknown correctly, you will receive 10 points • If you do not identify your unknown correctly, you will receive 0 points • There is NO partial credit • Failure in attempting to identify your unknown, will result in a 0 for the ENTIRE lab

  8. I. Exp. 16: Identification of Unknown Microorganisms • To Clean Up: • Remove tape from all tubes; put tubes in autoclave basket/racks • Discard all plates in the Biohazard Bag • Slides go in the “Sharps Container” • Remove tape from Slide Box • Fill out Course Evaluation and Index Card (advice you would like to give to the next semester BIO201students as to how to do well in the course)

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