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Control Gel. pCAT BamH1-HindIII partial digest. pCAT BamH1-HindIII-EcoR1. Note the partial digest has the linearized plasmid, the plasmid backbone and the CAT fragment. pCAT BamH1-HindIII. pBS BamH1-HindIII. pCAT BamH1. pCAT HindIII. pBS BamH1. pCAT uncut. pBS HindIII. 1 kb ladder.
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Control Gel pCAT BamH1-HindIII partial digest pCAT BamH1-HindIII-EcoR1 Note the partial digest has the linearized plasmid, the plasmid backbone and the CAT fragment pCAT BamH1-HindIII pBS BamH1-HindIII pCAT BamH1 pCAT HindIII pBS BamH1 pCAT uncut pBS HindIII 1 kb ladder pBS uncut CAT Plasmid backbone 3.0 kb CAT fragment 2.0 kb
Tuesday lab gel 1 1 kb ladder Emily/Rosario DJ Melanie/Laura 1 2 3 4 1 2 1 2 3 4 3.0 kb 2.0 kb Vector fragment CAT fragment
Tuesday Lab Gel 2 Susan/ Aleks/ Laura Elizabeth/ Vidi/Pavani 1 kb ladder Adam/Meredith Biff/Althea 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 Vector fragment 3.0 kb 2.0 kb CAT fragment
Wednesday Lab Gel 1 1-4 EB/MB: BS cut, CAT cut, BS uncut, CAT uncut 5-8 ASC/DD: BS cut, BS uncut, CAT cut, CAT uncut 9-13 SQ/TT: BS uncut, BS cut, CAT uncut, empty well, CAT cut 14-17 DS/BAM: BS cut, BS uncut, CAT cut, CAT uncut Marker EB/MB ASC/DD SQ/TT DS/BAM 1 1 kb ladder 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Vector fragment 3.0 kb 2.0 kb CAT fragment
Wednesday Lab Gel 2 1-4 DCP/WR: CAT cut, CAT uncut, BS cut, BS uncut 5-8 SJK/FMM: BS cut, BS uncut, CAT cut, CAT uncut 9-12 AW/JG/AF: BS uncut, BS cut, CAT uncut, CAT cut 13-16 AW/AB: CAT cut, CAT uncut, BS cut, BS uncut DCP/WR 3 1 kb ladder SJK/FMM AW/AB AW/JG/AF 1 2 5 6 7 8 4 9 10 11 13 14 15 16 12 Vector fragment 3.0 kb 2.0 kb CAT fragment