Chapter 17 Large-Scale Chromosomal Changes

1. Chapter 17 Large-Scale Chromosomal Changes Changes in Chromosome Number Changes in Chromosome Shape

2. Types of chromosome mutations all generated by natural mutagens—extreme temps, UV, chemicals, etc.

3. Euploidy • euploidy: change of chromosome number involving 1 or more whole genomes • autopolyploidy = doubling of genome from “wild type” (e.g., tetraploid from diploid, hexaploid from triploid) • allopolyploid = doubling of genome from hybrid of two distinct taxa (e.g., varieties, species, genera)

4. Balanced (normal meiosis) 2n = diploid 4n = tetraploid 6n = hexaploid 8n = octoploid and so on Unbalanced (abnormal meiosis) 1n = monoploid 3n = triploid 5n = pentaploid 7n = heptaploid [usually hybrids of ploidy levels on left] Examples of ploidy levels

5. Facts about polyploidy and allopolyploids • Uncommon in animals but abundant (ancient and ancestral?) in plants • Recent genetic research shows allopolyploids far more common than autopolyploids—different from theory • Many allopolyploids found with multiple origins—contrary to evolutionary paradigm of “single origin” for species

6. Polyploids often produce larger structures, e.g., guard cells, pollen,...

7. ...and fruits (e.g., tetraploid grapes)

8. Meiotic pairing in triploids—> unbalanced gametes (sterility) Mules have 63 chromosomes, a mixture of the horse's 64 and the donkey's 62. The different structure and number prevents the chromosomes from pairing up properly and creating successful embryos, rendering mules infertile.

9. Colchicine used to induce polyploidy

10. A famous natural allohexaploid: Bread wheat (Triticum aestivum)

11. Famous Examples of Allopolyploid Complexes • Appalachian Asplenium ferns—several diploids, triploid hybrids, several tetraploids • Domesticated coffee (Coffea arabica)--parentage documented through molecular cytogenetic “chromosome painting” • Dandelions, roses, blackberries--more complicated groups that also do agamospermy (sex without seeds)

12. Evolutionary consequences of polyploidy • polyploids often more physiologically “fit” than diploids in extreme environments • polyploids reproductively isolated from original ploidy levels, may eventually differentiate • allopolyploids commonly occupy ecological niches not accessible to parental types • opportunities for gene silencing or chromosomal restructuring without disastrous consequences

13. Monoploid plants grown in tissue culture

14. Summary • polyploids common in plants • autoploids formed by doubling of “wild type” genome, allopolyploids from doubling of hybrid • allopolyploids far more common than autopolyploids • polyploids often more “fit” than parent(s), often in niches different from parent(s) • opportunities for evolutionary change through gene silencing or chromosome restructuring

15. Facts about aneuploids • Rare in animals, always associated with developmental anomalies (if they survive) • Most well known examples in human genetic diseases • Common in plants, sometimes show phenotypes, sometimes not

16. Extra chromosome 21Down Syndrome

18. Figure 16-12 step 1 Meiotic nondisjunction = aneuploid products

19. Figure 16-12 step 2 Meiotic nondisjunction = aneuploid products

20. Figure 16-12 step 3 Meiotic nondisjunction = aneuploid products

21. Figure 16-12 step 4 Meiotic nondisjunction = aneuploid products

22. Figure 16-12 step 5 Meiotic nondisjunction = aneuploid products

23. Figure 16-12 step 6 Meiotic nondisjunction = aneuploid products

24. Trisomics of Datura (jimsonweed)

25. Large-Scale Chromosomal Changes Changes in Chromosome Structure

26. Types of chromosome mutations

27. Deletion loops in Drosophila genes missing from chromosome #2 #1 #2

28. Deletion loops in Drosophila

29. Deletion origin of “cri du chat” syndromesee hear: http://www.youtube.com/watch?v=TYQrzFABQHQ

30. Duplications following polyploidy in Saccharomyces

31. Inversions cause diverse changes breakpoints between genes 1 breakpoint between genes, 1 within gene breakpoints within 2 genes

32. Inversion loops at meiosis

33. Paracentric inversions can lead to deletion products

34. Paracentric inversions can lead to deletion products

35. Paracentric inversions can lead to deletion products

36. Paracentric inversions can lead to deletion products

37. Paracentric inversions can lead to deletion products

38. Paracentric inversions can lead to deletion products

39. Figure 16-29 step 1 Pericentric inversions can lead to duplication-and-deletion products

40. Figure 16-29 step 2 Pericentric inversions can lead to duplication-and-deletion products

41. Figure 16-29 step 3 Pericentric inversions can lead to duplication-and-deletion products

42. Figure 16-29 step 4 Pericentric inversions can lead to duplication-and-deletion products

43. Reciprocal translocation revealed by molecular cytogenetics

44. Figure 16-30 step 1 Chromosome segregation in reciprocal-translocation heterozygote

45. Figure 16-30 step 2 Chromosome segregation in reciprocal-translocation heterozygote

46. Figure 16-30 step 3 Chromosome segregation in reciprocal-translocation heterozygote

47. Variegation resulting from gene’s proximity to heterochromatin

48. Variegation in translocation heterozygote

49. Chloroplast rearrangements • Great evolutionary significance in reconstructing relationships among land plant lineages • Can easily be screened for by PCR amplification of “universal” chloroplast gene primer pairs flanking large regions of chloroplast Judd et al. (2002)

50. Chloroplast rearrangements • Major inversions found in certain groups of families of bryophytes, pteridophytes, gymnosperms and several groups of angiosperms • Loss of one copy of inverted repeat in a few families! • Numerous losses of certain introns across angiosperms (e.g., rpl2 in Cactaceae) • Differences in size of large single-copy region by expansion or contraction of intergenic spacers