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Are We There Yet? Zebrafish Nutrition

Are We There Yet? Zebrafish Nutrition. Stephen A. Watts, Louis D ’ Abramo, Susan Farmer, Lacey Dennis, Daniel Smith and Mickie L. Powell University of Alabama at Birmingham and Mississippi State University. Why Nutrition?. ZF have been used extensively as a drug and disease model

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Are We There Yet? Zebrafish Nutrition

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  1. Are We There Yet?Zebrafish Nutrition Stephen A. Watts, Louis D’Abramo, Susan Farmer, Lacey Dennis, Daniel Smith and Mickie L. Powell University of Alabama at Birmingham and Mississippi State University

  2. Why Nutrition? • ZF have been used extensively as a drug and disease model • ZF are a useful toxicology model • Despite their widespread use the nutritional requirements have not been determined

  3. Ingredients are practical • Possible anti-nutritional factors • No indication of quantities • Use of lakes and dyes • Use of preservatives

  4. There are no standards among diets in zebrafish studies!!

  5. Where do we start?

  6. Examples of Nutrition Studies

  7. Considerations in the Use of Formulated Diets • Age: Larval vs juvenile vs adult (breeding) • Physical form of the diet • Feed Frequency • Protein source/quality • Carbohydrate • Lipid and Fatty Acids • Gut Retention Time • Bacteria • Culture Container

  8. Methods • Fish were co-cultured with enriched rotifers for the first 5 days post hatch and fed Artemia for 11 days prior to stocking. • At 21 days post hatch 15 fish were stocked randomly into 2.8 liter tanks and maintained on a recirculating zebrafish system. • For each diet, fish were fed a ration in excess of 5% of their body weight per day, divided into two feedings. • Fish were photographed and weighed every two weeks to measure growth and adjust feed rations.

  9. UAB Z-12 DietFormulation

  10. Week 10 Artemia Gemma

  11. High BCI Low BCI

  12. Can one nutrient affect experimental outcomes?

  13. Protein • Mixed source vs sole source

  14. Amino Acid Analysis

  15. µCT of Mixed Protein and Soy Protein Diets

  16. Bone Alterations

  17. Conclusions • All diets supported growth and survival. However, commercial diets contain undefined ingredients. • The consequence: Nutrients and nutrient source affect outcomes. • Any health or disease-related outcomes will be affected by diet. • Interpretation of experimental results must be made within the context of a defined nutritional history, or lack thereof. • Microbiome?

  18. Acknowledgments • UAB NORC Aquatic Animals Research Core (NIH P30DK056336). • Jeff Barry, Adele Fowler, Chris Taylor, Michael Williams, Karen Jensen, Marlee Hayes • ACLAM

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