1 / 1

ABSTRACT Antimicrobial Activity of Lecythophora sp. strain 30.5, Endophytic Fungi

ABSTRACT Antimicrobial Activity of Lecythophora sp. strain 30.5, Endophytic Fungi Extract from Alyxia reinwardtii BL Some endophytic fungi isolated from Alyxia reinwardtii BL (Pulasari), one of these fungi identified as Lecythophora sp. strain 30.5. This endophytic fungi was

liuz
Download Presentation

ABSTRACT Antimicrobial Activity of Lecythophora sp. strain 30.5, Endophytic Fungi

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ABSTRACT Antimicrobial Activity of Lecythophora sp. strain 30.5, Endophytic Fungi Extract from Alyxia reinwardtii BL Some endophytic fungi isolated from Alyxia reinwardtii BL (Pulasari), one of these fungi identified as Lecythophora sp. strain 30.5. This endophytic fungi was cultivated and extracted its component substances with ethyl acetat, n-heksan, and n- butanol. The extracts of Lecythophora sp. strain 30.5 was evaluated for antimicrobial activity and determine Minimum Inhibitory Concentration (MIC) by using agar dilution method. Varying concentration of the extracts (between 1,2 x 102 ppm and 5,0 x 103 ppm) were prepared and 0,50 µL suspense of each test microorganism was spotted on the surface of the solidified extract-agar mixture, then incubated 37 ºC for 24-48 hours. The organism used in this study were Staphylococcus aureus ATCC 25923 dan Bacillus subtilis FNCC 0059 for Gram positive bacteria, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, dan Salmonella typhi for Gram negative bacteria, and Candida albicans for yeast. The ethyl acetat extract showed no antifungi activity and the MIC of this extract was 3,0 x 103 ppm against all the bacterial strains. The MIC of n-heksan and n-butanol extract were 5,0 x 103 ppm against Candida albicans, but had no antibacterial activity. Key words : endophytic fungi, Alyxia reinwardtii BL (Pulasari), Lecythophora sp. strain 30.5, antimicrobial activity, Minimum Inhibitory Concentration, agar dilution.

More Related