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pH 5.0

pH 5.0. pH 8.0. B. C. A*. A*B. T4 Ligase. 0. C. 1. B*. C. A. Pf1M1. B*C. T4 Ligase. 2. A. B. C*. 3. A. BstAP1. 4. S. S. E. E. F. S 1. S 2. F. E. S 1. S 2. F. S j-1. S j. S j+1. S j-1. S j. S j+1. S j-1. S j. S j+1. H 1. H 2.

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pH 5.0

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  1. pH 5.0 pH 8.0

  2. B C A* A*B T4 Ligase 0 C 1 B* C A Pf1M1 B*C T4 Ligase 2 A B C* 3 A BstAP1 4

  3. S S E E F S1 S2 F E S1 S2 F

  4. Sj-1 Sj Sj+1 Sj-1 Sj Sj+1 Sj-1 Sj Sj+1

  5. H1 H2

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