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Microbial source-tracking (MST): The good, the bad, and you!

Microbial source-tracking (MST): The good, the bad, and you!. Department of Biology and Microbiology University of Wisconsin - Oshkosh Oshkosh, WI 54901 Phone - (920) 424-1100 Fax - (920) 424-1101 E-mail - kleinhei@uwosh.edu http://bact.uwosh.edu/kleinhei.html. So why should I care?.

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Microbial source-tracking (MST): The good, the bad, and you!

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  1. Microbial source-tracking (MST): The good, the bad, and you! Department of Biology and Microbiology University of Wisconsin - Oshkosh Oshkosh, WI 54901 Phone - (920) 424-1100 Fax - (920) 424-1101 E-mail - kleinhei@uwosh.edu http://bact.uwosh.edu/kleinhei.html

  2. So why should I care?

  3. How Clean are you?

  4. Indicator organism results provide an assessment of the “RISK” of contacting a more pathogenic microorganism. What is “Risk”? A statistical probability of an event occurring in a population To assess ‘Real Risk’ we need more and better data.

  5. Drainfield Laterals Access Port Vent Drainfield Laterals Septic Systems Bather Load Tank Waterfowl Boaters Typar Leachfield Washed Stone Sources of Microbes

  6. None Spatial Sampling Physical Evaluations Weather Effects Antibiotic Sensitivity Testing Genetic Evaluations Viral or host-specific organisms detection Source Determination or Microbial Source Tracking (MST) Options

  7. Genetic Evaluations Human Avian Water Gel showing DNA from E.coli

  8. Genetic Evaluations

  9. Antibiotic Sensitivity Testing Resistance of E.coli isolates to Penicillin Control Avian Human

  10. 81.8 8.9 7.9 24.3 148.3 24.3 109.3 112.7 8.4 274.4 32.9 215.4 Outfalls 48” 24” 12”

  11. Unknown Lake, MI >2419 1980 1890 <1 230 620 210 Next year… no test over 100 MPN/100mL!!! 480 850 >2419 >2419 <1 >2419 >2419 >2419

  12. Avian Waste

  13. Spatial Evaluations

  14. Breakwall 36-48” 12.7 (±4.0) 45.9 (±30.6) 63.7 (±35.3) 109.5 (±90.3) 33.0 (±13.2) 12-20” 24-30” 89.7 (±55.2) 73.3 (±50.6) 226.6 (±132.5) 241.6 (±126.0) 56.0 (±15.8) 289.7 (±172.4) 136.9 (±62.8) 196.0 (±124.9) 98.2 (±57.2) Bridge 1 Inlet Bridge 2 Inlet 1 mile Beach B Beach A Shoreline Maslowski Park Beach Ashland, WI Spatial Sampling Results

  15. Land Use Evaluations

  16. Weather Effects

  17. “O” Antigens • Grouping of microorganisms based on presence of different somatic antigenic determinants • Reaction of specific “O” antigens with specific antisera or latex antibodies

  18. Norovirus Pathogens -Salmonella -Shigella -Campylobacter -Aeromonas • Novel Methods-Bacteroides -Real-time PCR • New twist on old methods ?-Enterococci

  19. Many MST ‘tools’ in the toolbox Each has advantages and disadvantages Each site and situation must be evaluated on an individual basis. Cost, objectives, and local concerns MUST factor in to decision-making process. Facilities available may dictate approach. Need large amount of data and sound approach no matter what method is chosen. Hold-on….some changes are coming! Conclusions

  20. Thank you!

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