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BSC1010C Final Lab Practical Review

Click on enter to see the answer. C lick enter again to move to next slide. BSC1010C Final Lab Practical Review. What was the substrate in the enzyme experiment?. Hydrogen Peroxide (H 2 O 2 ). What was the enzyme in the enzyme experiment?. catalase.

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BSC1010C Final Lab Practical Review

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  1. Click on enter to see the answer. Click enter again to move to next slide. BSC1010C Final Lab Practical Review

  2. What was the substratein the enzyme experiment? Hydrogen Peroxide (H2O2)

  3. What was the enzyme in the enzyme experiment? catalase

  4. What was the source of thisenzyme in the enzyme lab? Sheep’s blood

  5. What was/were the product(s)in the enzyme experiment? Water and oxygen gas

  6. What was the optimum pHfor the enzyme? pH 7

  7. Why is the enzyme less active at very high or very low pH? Enzyme is denatured and reaction stops

  8. What was the optimum temperaturefor the enzyme experiment? 40 C

  9. Why do you see little gas produced at low temperatures? Enzyme and substrate molecules are moving very slowly, so less enzyme-substrate collisions, So less reactions

  10. Why do you see little or no gas production at high temperatures? The enzyme, which is a protein, is denatured at high temperatures and reaction stops.

  11. What did we measure to determinethe rate of the enzyme reaction? Oxygen gas pressure – rate of oxygen gas production

  12. What was the control in the enzyme experiment? Distilled water in place of enzyme

  13. What was the inhibitor used in enzyme experiment? Copper sulfate (CuSO4)

  14. What should happen when the inhibitor is added in the enzyme experiment? Reaction should stop as the inhibitor is preventing the substrate and enzyme from coming together

  15. What happened to the enzyme reaction whenthe temperature was raised to 60 C? Reaction stopped because enzyme was denatured

  16. What is this plant? Coleus

  17. What is the name of the pigmentin these leaves that is water-soluble?What color is this pigment? Anthocyanin red

  18. What pigment is alcohol-soluble?What color is this pigment? Chlorophyll green

  19. What was used to indirectly detectphotosynthesis in these leaves? IKI

  20. What areas of the leaf carried out photosynthesis according to IKI test? Green areas (where chlorophyll was located)

  21. What is the name of the techniquethat uses this setup? chromatography

  22. What was this setup used toseparate? Plant pigments

  23. What is the genus of plant used to get these pigments from the leaves? Magnolia

  24. Name 3 things that determine the movement of the pigments up the paper. Size of pigment molecule (molecular weight) Solubility of pigment in the solvent H bonding of pigment to paper

  25. What causes the solvent tomove up the paper? Capillary action (adhesion + cohesion)

  26. What is used to set 100%in absorption spectrum experiment? acetone

  27. What is the equation forcell respiration? (balanced, please) C6H12O6 + 6 O2 6 CO2 + 6 H2O

  28. What is the balanced equationfor photosynthesis? 6 CO2 + 6 H2O  C6H12O6 + 6 O2

  29. Given cell respiration and photosynthesis,which happens at all times? Cell respiration

  30. Given cell respiration and photosynthesis, which happens only in the light? photosynthesis

  31. Where is photosynthesis occurringin the following graph? A B C D A and C (Photosynthesis is taking in CO2 so level falls)

  32. What happens to CO2 levels in the respirometerin the light? WHY? Decline Because photosynthesis is taking in CO2.

  33. What happens to CO2 levels in the respirometerin the dark? WHY? They go up. Photosynthesis is NOT occurring so only cell respiration which is producing CO2

  34. What genus of plant was used in the cell respiration – photosynthesis experiment? Viburnum

  35. What is the purpose of the beaker of water that was placed between the light source and the leaves? To act as a heat sink (absorb the heat given off by the light)

  36. What are the 2 typesof nuclear division? Mitosis and Meiosis

  37. In mitosis, how many cells are formed?What is their chromosome #? 2 cells Diploid number

  38. In meiosis, how many cells are formed?What is their chromosome #? 4 cells Haploid #

  39. What is the name of this region? Root cap

  40. What is the name of this region? Region of maturation or differentiation

  41. What is the name of this region? Region of elongation

  42. What is the name of this region? Apical meristem Or Region of cell division

  43. What is this stage? Is it a plant or animal cell? Anaphase Plant cell

  44. What stage is this?What is arrow pointing to? Telophase Cleavage furrow

  45. What is this phase?Plant or animal cell? Prophase Plant cell

  46. What is this phase?Plant or animal cell? Metaphase Plant cell

  47. What is this phase?What is arrow pointing to? Telophase Cell plate of plant cell

  48. What is this phase?Plant or animal cell? Anaphase Animal cell

  49. What is this phase of meiosis? Metaphase I

  50. What is this phase of meiosis? Anaphase I

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