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Scott W. Herke Manager, Genomics Facility College of Science Louisiana State University

Scott W. Herke Manager, Genomics Facility College of Science Louisiana State University. It’s a BIG topic!. Let’s trim it down. Core DNA Sequencing Facilities Technology & Operational Models for the Future. Scott W. Herke Manager, Genomics Facility College of Science

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Scott W. Herke Manager, Genomics Facility College of Science Louisiana State University

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  1. Scott W. Herke Manager, Genomics Facility College of Science Louisiana State University

  2. It’s a BIG topic! Let’s trim it down...

  3. Core DNA Sequencing Facilities Technology & Operational Models for the Future Scott W. Herke Manager, Genomics Facility College of Science Louisiana State University

  4. Genomics Facility @ College of Science, LSU

  5. Genomics Facility @ College of Science, LSU

  6. Where do we go from here?

  7. Where do we go from here?

  8. Where do we go from here?

  9. Sanger-Sequencing

  10. Sanger-Sequencing

  11. Sanger-Sequencing

  12. Life Cycles of MPS Technology

  13. Life Cycles of MPS Technology

  14. Life Cycles of MPS Technology

  15. Life Cycles of MPS Technology

  16. Life Cycles of MPS Technology

  17. ‘Still-born?’ MPS Platforms

  18. ‘Vanishing’ MPS Platforms

  19. IlluminaHiSeq • LifeTech 5500XL ‘Declining’ MPS Platforms

  20. Relative Output of ‘Bench-tops’

  21. Relative Output of ‘Bench-tops’

  22. Relative Output of ‘Bench-tops’

  23. Relative Output of ‘Bench-tops’

  24. Relative Output of ‘Bench-tops’

  25. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  26. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  27. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  28. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  29. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  30. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  31. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  32. Bench-top Platforms 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  33. 2012 Roadmap 1Assumes 75% wells occupied by ISPs & 35% loss to filters. 2 Assumes 75% of Manufacturer’s stated maximum value. 3 PE run for 200-bp total read; thus, read # is not doubled.

  34. Making ‘BIG’ Platforms small

  35. Making ‘BIG’ Platforms small

  36. Where do we go from here?

  37. Sequencing Technologies

  38. Sequencing Technologies

  39. Sequencing Technologies

  40. Sequencing Technologies

  41. Can Genome Centers run on Semi-conductors?

  42. Can Genome Centers run on Semi-conductors?

  43. Ion Family “6-month” Cycle

  44. Ion Family “6-month” Cycle

  45. Ion Family “6-month” Cycle • ....Proton IIQ1-2013

  46. Ion Family “6-month” Cycle

  47. Ion Family “6-month” Cycle

  48. Ion Family “6-month” Cycle

  49. Ion Family “6-month” Cycle Q1-2013 Q3-2012

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