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Experiment 4- Gene Expression Study in Arabidopsis Thaliana

Experiment 4- Gene Expression Study in Arabidopsis Thaliana. RNA Blot. Quantitative RT-PCR. Tools For Studying Differential Gene Activity. Microarrays. What Are The Major Differences Between DNA and RNA?. RNA Has a Uracil Instead of Thymine. RNA Has Ribose Sugar in Nucleotide.

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Experiment 4- Gene Expression Study in Arabidopsis Thaliana

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  1. Experiment 4- Gene Expression Study in Arabidopsis Thaliana

  2. RNA Blot Quantitative RT-PCR Tools For Studying Differential Gene Activity Microarrays

  3. What Are The Major Differences Between DNA and RNA?

  4. RNA Has a Uracil Instead of Thymine

  5. RNA Has Ribose Sugar in Nucleotide

  6. Eukaryotic RNAs Are Single-Stranded Polynucleotides Nucleotides Joined By Phosphodiester Bonds Like All Nucleic Acids Order 5’  3’ Leads to Function:Co-Linear With Gene Sequence

  7. RNA Has Intra-Strand Double Helices or Secondary Structure

  8. 2. Remove Genomic DNA Collecting plant materials (1-6) Precipitation & Binding (17-20) RNase-Free DNase I Lysis (7-11) 3. Electrophoresis Determining Quality of Isolated RNA Washing (21-32) Elute RNA (33-40) (12-16) Determining Concentration Total RNA Isolation Workflow 1. RNA Isolation

  9. Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System

  10. Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System

  11. Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System- Part II Leaf Silique Leaf Silique

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