1 / 8

Enzyme Lab Notebook Format

Explore enzyme function through spectrophotometry, color change observations, and calibration. Includes varied enzyme and substrate concentrations, temperature, pH, and exposure times experiments.

lraper
Download Presentation

Enzyme Lab Notebook Format

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Enzyme Lab Notebook Format • 1) Title/Date • 2) Pre-lab (Key Concepts, Equations, Materials) • 3) Purposes (2-3) • 4) Personal Account • 5) Discussion– only 1 question (on bottom of pg 3 of packet) • 6) Conclusion

  2. Lab 2: Enzyme catalysis Guaiacol + 2H2O2 tetraguaiacol + 8H2O (colorless) (brownish orange) Enzyme = peroxidase Substrate = peroxide (H2O2) How will we measure enzyme function? - Color change using a spectrophotometer peroxidase

  3. How does a spectrophotometer work? • Take home points • Light at a specific wavelength is sent through a liquid sample • Light passing through the sample is detected on the other side • To measure this accurately, we must calibrate (blank) the spec. • Calibrating the spec sets the range & “tells” the spec: • what 0% light is (darkness) • what 100% light is (all light passing through) • For our experiment we will see how the amount of light passing through changes as the reaction proceeds.

  4. Set wavelength to 500 nm • With the chamber empty, use left knob to adjust to 0% T • Insert tube #1 (blank) into spec & use right knob to adjust to 100% T • Pour #2 into #3 & begin timing immediately!! • Invert 2X quickly & place into spec • Record %T every 10 sec for 120 secs • From page 3, choose your experiment for tomorrow

  5. This weekend: -Work on Enzyme Lab Entry -Work on Pocket Mouse Signaling Packet -BOTH due on TUESDAY! • Enzyme Lab Overview • Only hypothesis/procedure/data from FRIDAY needs to be included. • Slope calculation for EACH of your four lines • ΔY / ΔX (Units = Y units / X units) • Discussion section (section #5) is only ONE question!

  6. Please put your LAB NOTEBOOK in the blue crate. • Please put your MOUSE SIGNALING PACKET in the appropriate homework file. • Make sure your name is on the packet!

  7. Unit 4 Test Review Session (Q&A) • TOMORROW (Wednesday) at 7:00am • TEST REMINDERS: • 1) Don’t forget about your “Potential FRQ” sheet…you got this on Tuesday, Nov. 24th • 2) Don’t forget about your “Math Practice” sheet…you got this in mid-November. Answers are posted online under Ch. 7 PPT notes

  8. ENZYME LAB OVERVIEW: • #1: Varied Concentration of Enzyme (peroxidase) • More enzyme = faster reaction • #2: Varied Concentration of Substrate (H2O2) • Less substrate = slower reaction • More substrate = faster reaction • Even more substrate = slower reaction • #3: Varied Environmental Temperature • Body temp = fastest rate • #4: Varied Environmental pH • pH 8 = slow reaction; pH 5 = slow reaction • #5: Varied Exposure Times to Boiling Water • Reaction stops completely when enzyme boiled

More Related