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Open Reading Frame (ORF). AUG. UAA. Generic Structure of a Eukaryotic mRNA. 3’-untranslated region (3’-UTR). 5’-untranslated region (5’-UTR). (AUUUA) n. (AAA) n. 7-Me-GpppG. stem-loop structures. cleavage & poly(A) addition site. stem-loop structures. cap. poly(A) tail.
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Open Reading Frame (ORF) AUG UAA Generic Structure of a Eukaryotic mRNA 3’-untranslated region (3’-UTR) 5’-untranslated region (5’-UTR) (AUUUA)n (AAA)n 7-Me-GpppG stem-loop structures cleavage & poly(A) addition site stem-loop structures cap poly(A) tail Initiation codon context -6 -5 -4 -3 -2 -1 +4 G C C A C C G Kozak (mammalian) consensus: -6 -5 -4 -3 -2 -1 +4 (GC-poor) A x A (U) S. cerevisiae (yeast) consensus:
7-methyl-guanine R1 and R2 = H or CH3 mRNA-3’-OH
• ATP and GTP • GTP
Gcn2 & other eIF2 kinases
eIF4G eIF3 Composed of 13 distinct gene products (eIF3a-eIF3m) mRNA 40S subunit