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3Glucose-6-P. 3Ru5P. +. Gln. F6P + E4P + R5P. 3PG. NH 3. +. 2 PEP + E4P + R5P. +. Serine. Tryptophan + pyruvate + GAP + CO 2. Aldolase. 2-keto-3-deoxy-arabinoheptulosonate-7-phosphate synthase. chorismate synthase. Anthranilate. Chorismate. Prephenate. p-hydroxybenzoate.
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3Glucose-6-P 3Ru5P + Gln F6P + E4P + R5P 3PG NH3 + 2PEP + E4P + R5P + Serine Tryptophan + pyruvate + GAP + CO2
chorismate synthase
Anthranilate Chorismate Prephenate p-hydroxybenzoate p-aminobenzoate Tryptophan Nictotinamide Phenylalanine Tyrosine Tetrahydrofolate Coenzyme Q
Ribose-5-phosphate Cost = 2 ATP
3 glucose + 5NADP+ + 2NAD+ + 2ATP serine + glutamine ---> Tryptophan + 5NADPH + 2NADH + 2ADP + 2Pi + GAP + pyruvate + 4CO2 + glutamate glutamine costs NADPH + 2NH4+ + 2ATP GAP + glutamate + ATP + 2NADH = serine 3 glucose + 4NADP+ + 2NH4+ + 4NAD+ + ADP + Pi ---> Tryptophan + 4NADPH + 4NADH + ATP + pyruvate + 4CO2
3 glucose + 2NAD+ + 4NADP+ + NH4+ + ATP ---> phenylalanine + R5P + 4NADPH + 2NADH + 4CO2 + ADP + Pi 3 glucose + 3NAD+ + 4NADP+ + NH4+ + ATP ---> tyrosine + R5P + 4NADPH + 3NADH + 4CO2 + ADP + Pi In animals tyrosine is made from phenylalanine by phenylalanine hydroxylase
Kynurenine N-formylkynurenine formamidase
N10-formyl-THF synthetase Loss of ATP! N10-formyl-THF
Kynurenine 3-hydroxykynurenine
So…Trp = alanine - ATP - NADPH + 3-hydroxyanthranilate If: Ala = 13 ATP Then: Trp = 12 ATP - NADPH + 3-hydroxyanthranilate
2-amino-3-carboxymuconate-6-semialdehyde 3-hydroxyanthranilate dioxygenase
+ NH3 Trp = 12 ATP - 2 NADPH - (NADH?) + NADH + -ketoadipate + NH3
-ketoadipate dehydrogenase Same mechanism as pyruvate dehydrogenase and -ketoglutarate dehydrogenase.