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Semen Evaluation

Semen Evaluation. ผศ .น.สพ. ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธุ์. Semen collection. Semen collector Bull handler Teaser handler. Semen collection. Artificial vagina. Semen collection. Electro-ejaculator. Semen collection : manual manipulation. Semen evaluation.

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Semen Evaluation

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  1. Semen Evaluation ผศ.น.สพ. ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธุ์

  2. Semen collection • Semen collector • Bull handler • Teaser handler

  3. Semen collection • Artificial vagina

  4. Semen collection • Electro-ejaculator

  5. Semen collection: manual manipulation

  6. Semen evaluation • Macroscopic evaluation • Microscopic evaluation • Special evaluation

  7. Macroscopic evaluation • VolumeBeef bull 5 -10 ml Dairy bull 10-15 ml

  8. 2. Consistency • Watery • Translucent • Milky • Creamy • Glue -like

  9. 3. Density • D = thin • DD = medium • DDD = thick

  10. 4. pH • Beef bull 6.7 • Elephant bull 7.04 • pH meter or pH paper

  11. 5. Contamination • Pus • Blood • hair • Urine

  12. 6.Color • Transparent gray • Evident gray • Strong white , Yellowish white • Yellow

  13. Microscopic Evaluation • 1.Mass Activity 10 X 0 = NO motility + = มีคลื่น แต่ไม่รุนแรงนัก ++ = เร็วและแรง หมุนช้า ๆ +++ = เป็นคลื่น หมุนรุนแรง

  14. 2. Motility • 10-40 X with Cover glass • Progressive movement • % motile sperm

  15. 3. Concentration • Haemocytometer method • Flow cytometry • Electronic particle counter • DNA assay • Computer-assistedsemen analyzers(CASA) • Turbidity -Photolectric colorimeter - Spectrophotometer

  16. Haemocytometer method • Formal saline dilute Boar 1: 50 Bull 1: 200 Stallion 1: 100- 40 X - (N x D x 250) / A mm3

  17. 4. Percentage of lived sperm • Eosin ,Nigrosin,Fast green analine blue • 100 x • 200-500 sperm

  18. 5. Sperm morphology • Dry preparation ---> Head of sperm Indian ink Eosin-Nigrosin Geimsa-stain Wright’s stain William stain

  19. Sperm morphology • Wet preparation ---> tail of sperm • Formal saline 1:2 ,1:5

  20. Classification of Abnormal Sperm • Primary abnormality ,Secondary abnormality • Major or Minor sperm abnormality

  21. Major sperm abnormality Proximal cytoplasmic dropletPyriform head

  22. Double headed sperm

  23. Strongly fold ,coiled tail ,coiled around the head

  24. Middle piece defects

  25. Maldeveloped or undelveloped

  26. Diadem defect or Pouch formation

  27. Minor sperm abnormality - Distal cytoplasmic droplets

  28. Tailess normal heads and detached sperm head

  29. Simple bent tail or Terminally coiled tail

  30. Narrow ,small ,Giant head

  31. Abaxial implantation

  32. Abnormal acrosome

  33. Special sperm evaluation • Metabolic reaction rates • Glycolysis • Respiration • Hydrogen ion concentration • Penetration hamster ova • Filtration • Sexing semen • etc

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