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ABX Pentra DF 120. Flexible hematology platform . For internal use only. ABX Pentra DF 120. Main features analysis Methods and technologies Pentra ML. Main features. Rack loader. Reagent compartment. Technical software. Processed tubes. Stat Sample Bay. ABX Pentra ML.
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ABX Pentra DF 120 Flexible hematology platform For internal use only Prepared by L.Castellane
ABX Pentra DF 120 Main features analysis Methods and technologies Pentra ML Prepared by L.Castellane
Main features Rack loader Reagent compartment Technical software Processed tubes Stat Sample Bay ABX Pentra ML Optional slide maker and stainer Prepared by L.Castellane
Main features • CBC, DIFF • IMmature Lymphocyte, Monocyte and Granulocyte • Optional and integrated slide maker and stainer • Expert Validation Station Prepared by L.Castellane
Main features 32 Parameters • CBC (12 parameters) • Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw • Differential count (12+20 parameters) • Lym, Mon, Neu, Eos, Baso, Aly, Lic (% and #) • IML, IMM, IMG (% and #) • Throughput (12O samples per hour) Prepared by L.Castellane
Main features • 5 reagents • ABX Diluent • ABX Basolyse • ABX Leucodiff • ABX Alphalyse or ABX Lysebio (Cyanide free) • ABX Cleaner • Unique blood control • ABX Difftrol - 3 levels • Target values on disk, multi-language package insert ABX reagents and controls are bar-coded and CE marked Batch number, level detection, small reagent volumes, waste level sensor Prepared by L.Castellane
Main features • 3 types of analyses (with random access) • CBC • DIFF • SPS External and internal bar-code reader Prepared by L.Castellane
ABX Pentra DF 120 Main features analysis Methods and technologies Pentra ML • CBC principles • 5 Diff – Double matrix Prepared by L.Castellane
Methods & technologies RBC/PLT - Impedance method HGB - Cyanmethemoglobin or cyanide free WBC - (Triangulation) -> WBC channel (impedance) -> BASO channel (Specific lyse + impedance) -> 5Diff channel (optical) Prepared by L.Castellane
Methods & technologies 5Diff Principles • 1. Cytochemistry • Lysing of red blood cells • Staining of nuclei and cytoplasmic granules of leukocytes • Fixing the leukocytes in their original state (48 hours post-draw stability) • 2. Double Hydrodynamic Sequential System (DHSS) • Flow cytometer with hydrodynamic focusing before and after the micro-aperture • 3. Impedance method • Measurement of the cell volume (“X” axis) • 4. Light absorbance • Internal structure (“Y” axis) Prepared by L.Castellane
Methods & technologies Eosinophils Large Immature Cells Neutrophils Lymphocytes Monocytes Atypical LYmphocytes Double Diff Matrix IMmature Granulocytic line IMmature Monocytic line IMmature Lymphocytic line Prepared by L.Castellane
Methods & technologies Metamyelocyte Myelocyte Promyelocyte Myeloblast IMmature Granulocytes IMG IMG% and # Identification of Granulocytic cell line count of the overall myelemia Prepared by L.Castellane
Methods & technologies Promonocyte Monoblast IMmature Monocyte IMM IMM % and # Identification of Monocytic cell line Count of the precursors of the monocytic line Prepared by L.Castellane
Methods & technologies IMmature Lymphocyte Lymphoblast Prolymphocyte IML IML % and # Identification of Lymphocytic cell line IML and ALY include all abnormal lymphocytic populations Prepared by L.Castellane
Methods & technologies • Routine identification and quantification of 3 immature cell lines involved in leucopoiesis: • Granulocytic line • Monocytic line • Lymphocytic line -> Reduction of the number of slide examinations -> Diagnostic and follow-up tool for rapid decision-making Prepared by L.Castellane
Methods & technologies Ne: (Neutro-eosino) Hyper-segmented neutrophils Hypo-granular eosinophils... ALY: (Atypical Lymphocytes) Atypical Lymphocytes Plasmocytes, hairy Cells... LIC: (Large Immature Cells) Blasts, myelocytes Promyelocytes Metamyelocytes... Mn: (Mono-neutro) Immature granulocytes Band cells... Nl: (Neutro-lympho) Hypo-segmented neutrophils Sezary cells, hairy cells... 1- Priority alarms 2- Separation alarms Prepared by L.Castellane
Methods & technologies No: (Noise) Platelet aggregates, NRBCs Lysis resistant RBCs Ln: (Left-neutro) Cellular debris Ll, Ll1: (Left-lympho) Platelet aggregates, NRBCs Rn: (Right-neutro) Immature granulocytes Rm: (Right-mono) Large monocytes, blasts 3- Zone alarms Prepared by L.Castellane
Methods & technologies CHRONIC MYELOID LEUKEMIA Pentra DF 120 result: NEU 25.4 % EOS 1.8 % LYM 5.1 % MON 8.6 % BAS 0.1 % IMG 49.3 % IMM 9.2 % IML 0.5 % ALY 1.3 % Prepared by L.Castellane
Methods & technologies ACUTE MONOBLASTIC & MONOCYTIC LEUKEMIA Pentra DF 120 result: NEU 11.1 % EOS 0.5 % LYM 9.3 % MON 47.9 % BAS 0.1 % IMG 4.1 % IMM 26.1 % IML 0.9 % ALY 1.1 % Prepared by L.Castellane
Methods & technologies T-CELL PROLYMPHOCYTIC LEUKEMIA Pentra DF 120 result: NEU 12.1 % EOS 0.4 % LYM 6.7 % MON 12.8 % BAS 0.4 % IMG 0.4 % IMM 2.0 % IML 7.4 % ALY 57.8 % Prepared by L.Castellane
ABX Pentra DF 120 Main features analysis Methods and technologies Pentra ML • Specs & functionality Prepared by L.Castellane
Specs & functionality Dedicated PC 17 inch flat colour screen Windows XP CD-ROM burner Automatic rerun Reflex testing Pre-diluted mode Cytology library Onboard user manual Prepared by L.Castellane
Specs & functionality • Mono or bi-directional connection: • ASTM format • Transfer of • Demographic data (blood type - man, woman, child) • Tests requested (CBC, CBC/DIFF, SPS) • Up to 3 ABX Hematology analyzers (Pentra range) can be connected • Query mode • Download capability Prepared by L.Castellane
Specs & functionality • User defined rules applied when : • receiving the worklist, • a sample has been analyzed. • _ Rerun capability • _ Reflex testing • _ Automatic validation • _ Messages, comments…. • Bio-technical validation with : • Patient history • Delta check • Result edition • Manual entry • Recalculation capability Prepared by L.Castellane
Result screens • Bio-technical validation with : • Patient report, run, re-run, patient history, graphs, alarms. All displayed on the same screen. • Delta check • Result edition • Manual entry • Recalculation capability 1- Title bar 2- Patient and Graphics area 3- Messages 4- Patient Comments 5- Technical Comments 6- Extended Diff 7- Result area 8- Report 9- Runs Prepared by L.Castellane
Specs & functionality Hematovision IV included A cell cytology library Prepared by L.Castellane
ABX Pentra 120 series Prepared by L.Castellane
ABX Pentra 120 series Prepared by L.Castellane
Thank you Prepared by L.Castellane