viral disease in aquarium fish

2. viral disease in aquarium fish S.salemi

3. introduction Fish • fishis any member of a paraphyletic group of organisms . . • Most fish are ectothermic ("cold-blooded") • There are a variety of environmental conditions. • Two Gill ماهی گارا روفا Fish love

4. Special fish • shape • Color • Size • varietyfood • In terms of behavior • Location Live

5. Development of a fish The life cycle of a fish contains 6 stages as generally recognized by science: Egg 2. Embry 3.Larva 4 .Juvenile 5.Adult 6.Senescence

6. Fish farming in Iranian mythology In Iranian mythology Jamshid, the first man to Fish farming assumed the payment was Pishdadi J. King. Iranian Mahykhanh place called the Fish farming. The artificial Fish farming in China began The old Fish farming in the world, the proliferation activity of rainbow trout Tasma Hyan education in 1301 than in 1338 started

7. Definition of ornamental fish

8. General Principles and History aquarium fish The first time the Chinese and Japanese with storageDevelopment and maintenance of ornamental fish in ponds Goldfish to beat 1000 years ago Fish aquarium in South East Asia firstOrnamental fish used in countriesWere European, including one fighter who neverOr Goldfish named. Germans for the first time in Europe reproduction, breeding and export of aquarium fish did. ? Was the first aquarium in 1819 by Pyps English. گلد فیش رد کپ

9. Aquarium • An aquarium (plural aquariums or aquaria) is a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept. Fishkeepers use aquaria to keep fish, invertebrates, amphibians, marine mammals, turtles, and aquatic plants. The term combines the Latin root aqua, meaning water, with the suffix -arium, meaning "a place for relating to.

10. The main factors in keeping aquarium fish diseases • Density of fish )Oxygen deficiency) • Physiologic and behavioral characteristics of fish • Factors water

11. Wonders of Creation اکسولوتل Axolotl ماهی انسان نما

12. How to identify a sick fish • major physical factors affecting patient diagnosis and identification of fish are: • languishing Fish • did not escape the effects of stimulating or frightening • Swimming abnormalseparate from other fish and throw them • keep your mouth open • rub the body wall or floor • stay in the water level

13. Aquarium Fish Diseases • Fungal (Nodular) (polydipsia) • Bacterial

14. (Hexamita) • Protozoan • Viral (DiscusPlague-Black Disease) (Fish Koi Herpes Virus)

15. Discus (fish) • Discus (Symphysodon spp.) are a genus of three species of cichlidfreshwater fish native to the Amazon River basin.[1] Discus are popular as aquarium fish and their aquaculture in sev.eral countries in Asia is a major industry.

16. Plague-Black Disease In September 1986, a new serious infection of certain cichlid species began appearing in wholesaler tanks all across the United States. • Airborne virus refers to any diseases which are caused by pathogens and transmitted through the air The body was black fish which appeared to have come in with Southeast Asian fish.such as: discus, angels, uarus, severums and oscars disease spread within days to all tanks containing the sensitive species suggesting airborne infection symptoms were found to have Hexamitiases Within a short time, numerous pet stores and fish breeders who handled these species were also experiencing severe disease problems.

17. viral diseases (DiscusPlague-Black Disease) What is black disease? What is discus plague? Black disease also referred to as Discus Plague disease caused by virus airborn happens kill all fish scratches Warden to the body

18. They stay just under the surface of water and breathe heavily epidemic spread rapidly mucus • up-and-down in the aquarium

19. Symptoms of DiscusPlague-Black Disease • being timid fish • The shy fish • up-and-down in the aquarium • Rapid breathing • Increased mucous secretions خطوط استرس بدن ماهی • Low Appetite fish • Smelly in the aquarium • increased mucus in the body

20. Secondary symptoms After a viral illness 1)Intestinal parasites and internal gills2) septicemia disease3) disease contemplating holes 4)black fish skin 5)Severe fin rot tail Treatment incresess Immune System aspirin, cold tablets, acid water, potassium

21. Complications • After Treatment 1)Sterilization Vnaza 2)Lack of spawning fish infemale 3)blind 4)thin 5) low growth

22. Koi fish The word koi comes from Japanese, simply meaning "carp". It includes both the dull grey fish and the brightly colored varieties . Various carp species were originally domesticated in East Asia, Koi varieties are distinguished by coloration. Some of the major colors are white, black, red, yellow, blue, and cream (used as food fish)

23. Differences from goldfish

24. koi herpesvirus (KHV): • herpes-like virus designated koi herpesvirus • (KHV) was f irst i sol ated in the USA in 1998. , but not confirmed until later in 1999 • KHV is a DNA-based virus mortalities in farmed fish • (KHV) in the family Alloherpesviridae Susceptible stages of the host All age groups of fish Species: koi common carp hybrids and goldfish Stability of the agent: ethyl alcohol at 30% for 20 minutes UV and 50°C for 1 minute

25. Koi Herpesvirus (KHV) is a very • damaging and easily spread virus that • affects common carp and carp varieties • such as koi carp. Outbreaks of the • disease occur in summer, when water • temperatures are high 25-23, and can kill • between 20 per cent and 100 per cent of • carp in a fishery. • gill necrosis virus • KHV epidemics • KHV remains active in water for at least 4 hours, • nfectious titre of KHV within 3 days

26. Environmental factors Necrotic gills in common carp • water temperature • virulence of the virus, • condition of the fish • population density • stress factors (e.g. transportation, spawning, poor water quality) • THE disease is temperature dependent (occurring between 16 and 25°C ) • high mortality at 28°C • low temperatures may be reservoirs of the virus • viral DNA was detected in the fish by PCR at 13°C, 15°C and 28°C

27. Target organs and infected tissue: • Gill, kidney, and spleen • Vectors: • Water is the major abiotic vector. However, animate vectors (e.g. other fish species, parasitic • invertebrates and piscivorous birds and mammals) and fomites may also be involved in transmission.

28. Life cycle • the gills are the major portal of virus entry in carp • However, a more recent skin entry for KHV systemic spread of the virus: skin and gills to the internal organs and high levels of KHV DNA have been detected in kidney, spleen, liver and gut tissue early stages of KHV infection: mucus

29. Transmission mechanisms ‘vertical • The mode of transmission of KHV is horizontal but ‘egg-associated. direct (fish to fish) faeces, urine, gills and skin mucus Virulent virus is shed via • infectious virus was continuously shed for a longer period • from infected common carp at 16°C than those at 23°C or 28°C can be rapid: temperatures (23–25°C) less rapid: below 23°C The disease may manifest itself in 3 days fish to a pond 8–21 days

30. Symptoms of KHV • Symptoms of KHV include: • Gill mottling necrose • Red and white patches appearing on gills • Bleeding gills • Sunken eyes • Pale patches • Blisters • tissue, often with secondary infection of bacteria and fungi. • loss • balance, loss of mucus resulting in dry

31. Diagnostic methods • Detection of KHV by PCR THE BEST • ELISA not been extensively reported • [IF] • TEM no treatment

32. refrens • 1)Manual of Diagnostic Tests for Aquatic Animals 2012 . KOI HERPESVIRUS DISEASE • Chapter 2.3.6. — Koi herpesvirus disease . • 2)Pokorova.D.etal.2005. Current knowledge on koi herpesvirus (KHV), Vet. Med. – Czech, 50, 2005 (4): 139–147 , Review Article • 3) The Animal Welfare Act 2006 . Koi Herpesvirus (KHV) • Disease., • 4)www.wikipedia • 5)www.enviroment-agency.gov.uk

33. Thank you for your attention