1 / 23

Research regarding in vitro propagation of some Lycium barbarum and Lycium chinense hybrids

Research regarding in vitro propagation of some Lycium barbarum and Lycium chinense hybrids. Authors names : C ătălina Nicolae Adrian Peticilă PhD. student Oana Venat Assoc.prof.dr . Adrian Asănică Popescu Dan. Coordinator Professor : Assoc.prof.dr . Adrian Peticil ă.

maddox
Download Presentation

Research regarding in vitro propagation of some Lycium barbarum and Lycium chinense hybrids

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Research regarding in vitro propagation of some Lyciumbarbarumand Lyciumchinensehybrids Authors names: Cătălina Nicolae Adrian Peticilă PhD. student Oana Venat Assoc.prof.dr. Adrian Asănică Popescu Dan Coordinator Professor: Assoc.prof.dr. Adrian Peticilă International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  2. Goji berry – a superfruit Lycium barbarum and Lycium chinense are spinous shrubs in the nightshade Solanaceaefamily. Their fruits have been used in traditional chinese medicine for over 2.300 years. Some of the health benefits include: antioxidant, anti-aging, regulator of the immune system, inhibition of tumoral growth and anti-diabetes effect. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  3. Goji berry – a superfruit Goji are considered superfruits for their high content of nutrients Polysaccharides B Vitamins Thiamine Riboflavin Piridoxine 5-8% xylose, glucose, arabinose, rhamnose, mannose, galactose Vitamin C 42 mg/100 g Carotenoids The most abundant - zeaxanthin 18 aminoacids Of which 8 are essential International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  4. Propagation of Lyciumbarbarum In Romania, studies on propagation of Lycium barbarummainly regarded seeds and cuttings, but also in vitro cultures. Generative propagation offers a diversity of biological material, therefore fruits may differ in terms of size, color, shape, and biochemical composition. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  5. Micropropagation of Lyciumbarbarum • Micropropagationtehniques allow the production of large number of plants in relatively short period of time. • Micropropagation of Lyciumbarbarumhas been studied by numerous authors and explant sources used include shoot tips, nodal segments, stem axillary buds, leaf segments and root segments, on different media types with wide range results. 1 2 3 4 6 5 Lyciumbarbarum – photos taken during in vitro stages: 1- root development, 2,3 – callus mass 3,4 – in vitro seedlings, 5 – root buds

  6. Materials and Methods Culture initiation: Seeds from 15 hybrids of Lyciumbarbarumand Lyciumchinensewere germinated aseptically. Prior to sterilization, seeds were immersed in 45°C water (warm water). International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  7. Materials and Methods Culture initiation: Seed sterilization: • 0,4 % Flint fungicide for 10 minutes; • 3 rinses in sterile bidistilled water, 5 minutes each; • 12 % Domestoscommercial solution (sodium hypoclorite 4,5 %) 15 minutes; • 3 rinses in sterile bidistilled water, 5 minutes each. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  8. Materials and Methods Culture conditions a. IN VITRO • Seeds were taken from a range of hybrid collection and were inoculated for on two different culture mediums: Murashigeet. Skoog (MS) and Gamborg (B5), both without growing hormones. Number of seeds/berry was not standardized, the condition of growth, ripening and number of berries for each hybrid has varied. • Seeds were incubated in the growth room, at 22 °C, 50 % humidity and under dark conditions untilgermination. • After germination, seedlings were kept at 16 hours photoperiod and 9.280 lxlight intensity. Germination observations were made every two days during a period of 30 days and measurements were made every 2 weeks. Data shown was germination rate, seedlings growth: lenght, leaves and internodes. • After a complete in vitro micro propagation cycle, most of hybrids were encapsulated in artificial seeds for testing their resistance after dormancy period in cool environment at 3-5 °C in dry/liquid medium (data not shown) for storing. b. SOIL For each hybrid, a number of seeds were germinated nonaseptically, in seedlings substrate and kept at room temperature, in lab environment, without particular conditions. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  9. Materials and Methods Culture conditions a. IN VITRO b. SOIL International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  10. Results and discussions I. Germination percentage In vitro In vitro: average germination percentage was 68,82 %. GI-1 ( 100%), CIIR7P5 (90%) and CI (88,23%) had the highest germination percentage. Only 3 hybrids - BI, BI liberand DI presented less 50% germination percentage. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  11. Results and discussions I. Germination percentage soil Soil germination: Average germination percentage was 33,52 %.CI (83,3 %) and FII (66,6 %) had the best germination rate. 11 hybrids- EI, AI, BI, BI liber, CI- 1, CI I- R7P1, CII - R7P3, CII - R7P5, DI- 1, GI- 1 and G II had a germination percentage under 50 %. DI had 0 % germination. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  12. Results and discussions I. Germination percentage Comparison between in vitro and soil propagation all seeds germinated in vitro conditions showed a higher germination percentage than the ones germinated in soil International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  13. Results and discussions I. Germination percentage MS (-) Culture medium The average germination percentage on MS (-) medium was 61,28 %. EI-1, CII-R7P5 and GI-1 report a 100% of germination percentage on MS medium and 66,67% of hybrids had a germination percentage over 50 %. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  14. Results and discussions I. Germination percentage B5 (-) Culture medium The average germination percentage on Gamborg B5 (-) medium was 76,36 %. AI, CI, DI-1, GI-1 and GII reported a 100% of germination on B5 (-) medium and 86,7% of hybrids had a germination percentage over 50%. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  15. Results and discussions I. Germination percentage MS(-) and B5 (-) Culture medium Comparation between mediums reveals that B5(-) medium provided a superior germination rate and promoted a much better growth environment for seeds. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  16. Results and discussions I. Germination percentage on soil and culture medium MS and B5 medium E I, A I, B I liber, C I, D I – 1, G II, F II showed a higher germination percentage on B5 medium; E I- 1, C II R7P1, C II R7P3, C II R7P5 showed a higher germination percentage on MS medium; B I, C I – 1, D I, GI – 1 – recorded no differences on the two growing mediums. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  17. Results and discussions II. Seedlings growth on MS(-) culture medium (average results) One month after inoculation, 5 hybrids did not have growth to report (BI liber, CII- R7P3, CII-R7P5, DI, DI-1); the best result in terms of growing is shown by GI- 1 with an average of 2,17 cm; from the hybrids that had a maximum germination rate on MS(-) - EI-1, CII-R7P5 and GI-1- only GI-1 reported a better growth; the average growth rate is 1,10 cm. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  18. Results and discussions II. Seedlings growth on B5(-) culture medium (average results) All hybrids reported growth on Gamborg B5 medium. The highest growth was recorded by CI – 1 (2,9 cm), CII R7P3 (2,6 cm) and E I (2,5 cm). The average seedling growth was 1,73 cm on Gamborg B5 Medium. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  19. Results and discussions II. Seedlings length growth on MS(-) and B5(-) During the first month after inoculation, all hybrids recorded better growth in terms of length on B5 medium, with an 1,73 cm average of growing, while on MS medium, the average growth was 1, 10 cm. International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  20. Results and discussions II. Seedlings growth Differences between the two growing medium on two hybrids, after one monthfrom germination. E I C I On left – MS medium; On right – B5 medium On left – B5 medium; On right – MS medium International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  21. Results and discussions III. Seedlings foliage growth on MS(-) and B5(-) Five (5) hybrids did not have foliage growth on MS (-) to report (BI liber, CII R7P3, C II R7P5, DI, DI – 1) . Foliage growth on B5(-) for AII, CI-1, CII R7P1 was double than MS (-). Four hybrids have shown no difference on the two growing mediums (EI, EI- 1, CI, GI-1).; F II was the only hybrid to show a better foliage growth on MS (-) International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  22. Conclusions and recommendations • In vitro conditions provided to be superior in terms of germination percentage. • On average, comparing the two growing mediums, seeds inoculated on Gamborg B5 (-) medium shown a higher germination percentage, higher seedling and foliar growth compared to MS (-) • CI-1, CII R7P3, EI-1, GI-1 responded overall better then other hybrids to micropropagation • The rate of germination and the potential of explants depends in a high percentage on seed maturation and pollination (data not tracking)(tbd) • The potential of in vitro propagation is higher on this species because of one particular aspect of it biology: there is a considerable activity on explant to generate new plants form dormant buds on the roots (genetic behavior). International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

  23. References • Amalgase H., Farnsworth N.R., 2011 A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lyciumbarbarum fruit (Goji). Food research International 44, 1702-1717. • Asănică A., 2017 – Vertical training of berry shrubs, Total Promotion Publishing House. • AsănicăA., Tudor V., Teodorescu R.I., Iacob A., Zolotoi V., Tudor A., 2016. Results on hardwood cuttings propagation of some Lycium sp. Genotypes. Frut Growing Research, Vol XXXII. • Cho-Wing Sze S., Song JX., Wong RN., Feng YB, Ng TB, Tong Y., Zhang KY., 2008. Application of SCAR (sequence characterized amplified region) analysis to authenticate Lyciumbarbarum (wolfberry) and its adulterants. Biotechnol. Appl. Biochem., 51, 15-21. • DănăilăGuidea SM, RV Dobrinoiu, L. Vişan, R.C. Toma, 2015. Protocol for efficient in vitro multiplication of Lyciumbarbarum L. by direct organogenesis. Scientific Bulletin. Series F. Biotechnologies, Vol. XIX • Fira A., N. Joshee, V. Cristea, M. Simu, M. Hârţa, D. Pamfil, D. Clapa, 2008. Optimization of micropropagation protocol for Goji Berry (Lyciumbarbarum L.). Bulletin USAMVM Horticulture 73 (2). • Kocyigit E., Sanlier N., 2017. A review of Composition and Health Effects of Lyciumbarbarum. International Journal of Chinese Medicine. 1 (1): 1-9. • PotteratO., 2010. Goji (Lyciumbarbarum and Lyciumchinense): Phytochemistry, pharmacology and safety in the perspective of traditioal uses and recent popularity. Planta Med. 76 (1): 7-9. • Redgwell, R. J., Curti, D., Wang, J., Dobruchowska, J. M., Gerwig, G. J., Kamerling, J. P., & Bucheli, P. (2011). Cell wall polysaccharides of Chinese Wolfberry (Lycium barbarum): Part 1. Characterisation of soluble and insoluble polymer fractions. Carbohydrate Polymers, 84(4), 1344-1349 • Tudor V., Asănică A., Teodorescu I.R., Gîdea M., Tănăsescu Constantin, Tudor A.D., Ţiu J.V., 2017. Germination capacity of some LyciumbarbarumL. and LyciumchinenseMill. biotypes seeds. Romanian Biotechnological Letters. Vol. 22, No. 1 • Images • http://perdayhealthsolution.blogspot.ro/2018/01/goji-8.html • http://www.lubera.co.uk/blog/plants/turgidus-the-large-sweet-goji-berry • http://www.specialtyproduce.com/produce/Goji_Berries_6465.php International Student Symposium "Hortus Academicus“ April 27, 2018 - Bucharest, Romania

More Related